apoTrpR
MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA LGTRVRIVEE LLRGEMSQRE LKNELGAGIA TITRGSNSLK AAPVELRQWL EEVLLKSD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 54.8 % (702 of 1281) | 56.1 % (377 of 672) | 47.9 % (235 of 491) | 76.3 % (90 of 118) |
Backbone | 73.1 % (468 of 640) | 86.2 % (187 of 217) | 60.5 % (193 of 319) | 84.6 % (88 of 104) |
Sidechain | 42.6 % (317 of 744) | 41.8 % (190 of 455) | 45.5 % (125 of 275) | 14.3 % (2 of 14) |
Aromatic | 15.5 % (9 of 58) | 24.1 % (7 of 29) | 0.0 % (0 of 27) | 100.0 % (2 of 2) |
Methyl | 44.9 % (61 of 136) | 52.9 % (36 of 68) | 36.8 % (25 of 68) |
1. apo TrpR
MAQQSPYSAA MAEQRHQEWL RFVDLLKNAY QNDLHLPLLN LMLTPDEREA LGTRVRIVEE LLRGEMSQRE LKNELGAGIA TITRGSNSLK AAPVELRQWL EEVLLKSDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 5.7, Details 10 mM Tris-HCl buffer pH 5.7, 100 mM NaCl, 5 mM DTT and 0.02 % NaN3
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | apo TrpR | [U-100% 13C; U-100% 15N] | 0.5 mM | |
2 | Tris-HCl buffer | natural abundance | 10 mM | |
3 | NaCl | natural abundance | 100 mM | |
4 | DTT | natural abundance | 5 mM | |
5 | NaN3 | natural abundance | 0.02 % | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |