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BMRB: 26853

5KQC

Identification and structural characterization of LytU

Authors

Tossavainen, H., Raulinaitis, V., Permi, P.

Assembly

LytU

Entity

1. LytU (polymer, Thiol state: not present), 145 monomers, 16247.92 Da Detail

GSKWEDFFRG SRITETFGKY QHSPFDGKHY GIDFALPKGT PIKAPTNGKV TRIFNNELGG KVLQIAEDNG EYHQWYLHLD KYNVKVGDRV KAGDIIAYSG NTGIQTTGAH LHFQRMKGGV GNAYAEDPKP FIDQLPDGER SLYDL

Formula weight

16247.92 Da

Source organism

Staphylococcus aureus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 86.2 %, Completeness (bb): 81.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (1467 of 1701)	93.3 % (828 of 887)	75.5 % (499 of 661)	91.5 % (140 of 153)
Backbone	81.4 % (697 of 856)	93.7 % (283 of 302)	69.0 % (287 of 416)	92.0 % (127 of 138)
Sidechain	92.0 % (893 of 971)	93.2 % (545 of 585)	90.3 % (335 of 371)	86.7 % (13 of 15)
Aromatic	79.7 % (153 of 192)	83.3 % (80 of 96)	75.5 % (71 of 94)	100.0 % (2 of 2)
Methyl	98.4 % (126 of 128)	98.4 % (63 of 64)	98.4 % (63 of 64)

1. LytU

GSKWEDFFRG SRITETFGKY QHSPFDGKHY GIDFALPKGT PIKAPTNGKV TRIFNNELGG KVLQIAEDNG EYHQWYLHLD KYNVKVGDRV KAGDIIAYSG NTGIQTTGAH LHFQRMKGGV GNAYAEDPKP FIDQLPDGER SLYDL

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	LytU	[U-13C; U-15N]	0.5 mM
2	Bis-Tris	natural abundance	20 mM
3	H2O	natural abundance	93 %
4	D2O	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.67 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.67 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl carbon	0.0 ppm	internal	indirect	0.2514495
¹H	water	protons	4.67 ppm	internal	direct	1.0
¹⁵N	liquid anhydrous ammonia	nitrogen	0.0 ppm	internal	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Release date

2016-07-13

Citation

Identification and structural characterization of LytU, a unique peptidoglycan endopeptidase from the lysostaphin family
Raulinaitis, V., Tossavainen, H., Aitio, O., Juuti, J.T., Hiramatsu, K., Kontinen, V., Permi, P.
Sci. Rep. (2017), 7, 6020-6020, PubMed 28729697 , DOI 10.1038/s41598-017-06135-w , Abstract

Entries sharing articles BMRB: 2 entries Detail

  BMRB: 30130 released on 2016-11-10
    Title Identification and structural characterization of LytU
  BMRB: 30131 released on 2016-11-10
    Title Identification and structural characterization of LytU

Related entities 1. LytU, : 1 : 1 : 1 : 14 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC