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BMRB: 26862

Stress Response Peptide-2; Manduca Sexta

Authors

Schrag, L.G., Cao, X., Herrera, A.I., Wang, Y., Jiang, H., Prakash, O.

Assembly

Stress Response Peptide-2

Entity

1. Stress Response Peptide-2 (polymer, Thiol state: all disulfide bound), 25 monomers, 2753.122 Da Detail

FGVKDGKCPS GRVRRLGICV PDDDY

Formula weight

2753.122 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS19:SG

Source organism

Manduca sexta

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.4 %, Completeness (bb): 96.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	88.4 % (130 of 147)	88.4 % (130 of 147)
Backbone	96.2 % (50 of 52)	96.2 % (50 of 52)
Sidechain	84.2 % (80 of 95)	84.2 % (80 of 95)
Aromatic	100.0 % (9 of 9)	100.0 % (9 of 9)
Methyl	80.0 % (8 of 10)	80.0 % (8 of 10)

1. Stress Response Peptide-2

FGVKDGKCPS GRVRRLGICV PDDDY

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K

#	Name	Isotope labeling	Type	Concentration
1	Stress Response Peptide-2	natural abundance		2.2 mM

Release date

2016-07-26

Citation

Solution Structure and Expression Profile of an Insect Cytokine: Manduca sexta Stress Response Peptide-2
Schrag, L.G., Cao, X., Herrera, A.I., Wang, Y., Jiang, H., Prakash, O.
Protein Pept. Lett. (2017), 24, 3-11, PubMed 27903232 , DOI 10.2174/0929866524666161121142840 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 30306 released on 2017-07-05
Title Cytokine-like Stress Response Peptide-2 in Manduca Sexta

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr26862_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:8:CYS:SG	1:19:CYS:SG	unknown	unknown	n/a

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr26862_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	148		100.0 (chain: 1, length: 25)
1	Chemical shift references	chemical_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 25, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 148
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
7	LYS	HZ1	7.503
7	LYS	HZ2	7.503
7	LYS	HZ3	7.503

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	147	129	87.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	52	50	96.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	95	79	83.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	10	8	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	9	9	100.0

Keywords 2D Nuclear Magneitc Resonance, Cytokine, Epidermal Growth Factor, Hemolymph, Insect Immunity, Prophenoloxidase

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Found 1 Document (0.265 seconds)

BMRB: 26862

Sample

Entries sharing articles BMRB: 1 entries Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail