BMRB: 26934

Solution Structure of first stem-loop of Escherichia coli DsrA RNA

Authors

Wu, P., Wu, J., Shi, Y.

Assembly

first stem-loop of DsrA RNA

Entity

1. first stem-loop of DsrA RNA (polymer, Thiol state: not present), 23 monomers, 7380.323 Da Detail

GGACAUCAGA UUUCCUGGUG UCC

Formula weight

7380.323 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.0 %, Completeness (bb): 94.1 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	93.0 % (345 of 371)	96.6 % (196 of 203)	90.3 % (140 of 155)	69.2 % (9 of 13)
Suger, PO4	94.1 % (238 of 253)	97.8 % (135 of 138)	89.6 % (103 of 115)
Nucleobase	90.7 % (107 of 118)	93.8 % (61 of 65)	92.5 % (37 of 40)	69.2 % (9 of 13)
Aromatic	89.6 % (95 of 106)	92.5 % (49 of 53)	92.5 % (37 of 40)	69.2 % (9 of 13)

1. RNA (23-MER)

GGACAUCAGA UUUCCUGGUG UCC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	RNA_(23-MER)	natural abundance		0.7 mM
2	sodium phosphate	natural abundance		10 mM
3	sodium chloride	natural abundance		50 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
4	RNA_(23-MER)	natural abundance		0.7 mM
5	sodium phosphate	natural abundance		10 mM
6	sodium chloride	natural abundance		50 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
7	RNA_(23-MER)	[U-100% 13C; U-100% 15N]		0.7 mM
8	sodium phosphate	natural abundance		10 mM
9	sodium chloride	natural abundance		50 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
10	RNA_(23-MER)	[U-100% 13C; U-100% 15N]		0.7 mM
11	sodium phosphate	natural abundance		10 mM
12	sodium chloride	natural abundance		50 mM

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Release date

2016-11-03

Citation

The important conformational plasticity of DsrA sRNA for adapting multiple target regulation
Wu, P., Liu, X., Yang, L., Sun, Y., Gong, Q., Wu, J., Shi, Y.
Nucleic Acids Res. (2017), 45, 9625-9639, PubMed 28934467 , DOI 10.1093/nar/gkx570 , Abstract

Related entities 1. first stem-loop of DsrA RNA, : 1 entities Detail

Experiments performed 14 experiments Detail

Chemical shift validation 3 contents Detail