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Found 1 Document (1.343 seconds)

BMRB: 27057

Chemical Shift Assignment of the PP1 and Microtubule binding domain of human KNL-1

Authors

Bajaj, R., Page, R., Peti, W.

Assembly

N terminal 80 residues of of KNL-1

Entity

1. N terminal 80 residues of of KNL-1 (polymer, Thiol state: all free), 84 monomers, 9490.531 Da Detail

GAMGMDGVSS EANEENDNIE RPVRRRHSSI LKPPRSPLQD LRGGNETVQE SNALRNKKNS RRVSFADTIK VFQTESHMKI VRKS

Formula weight

9490.531 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 41.9 %, Completeness (bb): 64.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	41.9 % (406 of 970)	18.0 % (93 of 518)	64.9 % (235 of 362)	86.7 % (78 of 90)
Backbone	64.7 % (321 of 496)	48.5 % (82 of 169)	65.2 % (161 of 247)	97.5 % (78 of 80)
Sidechain	29.3 % (162 of 553)	3.2 % (11 of 349)	77.8 % (151 of 194)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 28)	0.0 % (0 of 14)	0.0 % (0 of 14)
Methyl	50.0 % (35 of 70)	5.7 % (2 of 35)	94.3 % (33 of 35)

1. KNL-1

GAMGMDGVSS EANEENDNIE RPVRRRHSSI LKPPRSPLQD LRGGNETVQE SNALRNKKNS RRVSFADTIK VFQTESHMKI VRKS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 283 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	KNL-1	[U-98% 13C; U-98% 15N]	0.6 mM
2	HEPES	natural abundance	10 mM
3	Sodium cloride	natural abundance	50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 2 Detail

Release date

2017-03-26

Citation

KNL1 Binding to PP1 and Microtubules Is Mutually Exclusive
Bajaj, R., Bollen, M., Peti, W., Page, R.
Structure (2018), 26, 1327-11336, PubMed 30100357 , DOI 10.1016/j.str.2018.06.013 , Abstract

Related entities 1. N terminal 80 residues of of KNL-1, : 2 : 6 entities Detail

Interaction partners 1. N terminal 80 residues of of KNL-1, : 41 interactors Detail

More details for 41 interactors identified by 17 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr27057_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr27057_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	388		96.4 (chain: 1, length: 84)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 84, Sequence coverage: 96.4%
- Total number of assignments
  - ¹H chemical shifts: 78
  - ¹³C chemical shifts: 232
  - ¹⁵N chemical shifts: 78
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	518	78	15.1
¹³C chemical shifts	362	232	64.1
¹⁵N chemical shifts	100	78	78.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	169	78	46.2
¹³C chemical shifts	168	81	48.2
¹⁵N chemical shifts	80	78	97.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	349	0	0.0
¹³C chemical shifts	194	151	77.8
¹⁵N chemical shifts	20	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	38	0	0.0
¹³C chemical shifts	38	33	86.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	14	0	0.0
¹³C chemical shifts	14	0	0.0

Keywords KNL-1, Kinetochore, Microtubule binding, Protein phosphatase1, cell cycle

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Found 1 Document (1.343 seconds)

BMRB: 27057

Sample

Chemical shift reference

Chemical shift reference

Related entities 1. N terminal 80 residues of of KNL-1, : 2 : 6 entities Detail

Interaction partners 1. N terminal 80 residues of of KNL-1, : 41 interactors Detail

Experiments performed 6 experiments Detail

Instrument

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Chemical shift validation 3 contents Detail