NMR study of non-structural proteins part III: 1H, 13C, 15N resonance assignment of macro domain from Chikungunya virus (CHIKV)
MKHHHHHHAP SYRVKRMDIA KNDEECVVNA ANPRGLPGDG VCKAVYKKWP ESFKNSATPV GTAKTVMCGT YPVIHAVGPN FSNYSESEGD RELAAAYREV AKEVTRLGVN SVAIPLLSTG VYSGGKDRLT QSLNHLFTAM DSTDADVVIY CRDKEWEKKI SEAIQMRT
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.5 % (1526 of 1896) | 79.0 % (776 of 982) | 81.8 % (607 of 742) | 83.1 % (143 of 172) |
Backbone | 89.6 % (889 of 992) | 90.0 % (305 of 339) | 89.9 % (443 of 493) | 88.1 % (141 of 160) |
Sidechain | 73.5 % (780 of 1061) | 73.3 % (471 of 643) | 75.6 % (307 of 406) | 16.7 % (2 of 12) |
Aromatic | 0.7 % (1 of 142) | 0.0 % (0 of 71) | 1.4 % (1 of 69) | 0.0 % (0 of 2) |
Methyl | 97.1 % (167 of 172) | 95.3 % (82 of 86) | 98.8 % (85 of 86) |
1. CHIKV macro domain
MKHHHHHHAP SYRVKRMDIA KNDEECVVNA ANPRGLPGDG VCKAVYKKWP ESFKNSATPV GTAKTVMCGT YPVIHAVGPN FSNYSESEGD RELAAAYREV AKEVTRLGVN SVAIPLLSTG VYSGGKDRLT QSLNHLFTAM DSTDADVVIY CRDKEWEKKI SEAIQMRTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CHIKV macro domain | [U-99% 15N] | 0.6 mM | |
2 | soduim chloride | natural abundance | 20 mM | |
3 | Hepes | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | CHIKV macro domain | [U-99% 13C; U-99% 15N] | 0.6 mM | |
5 | soduim chloride | natural abundance | 20 mM | |
6 | Hepes | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | CHIKV macro domain | natural abundance | 0.3 mM | |
8 | soduim chloride | natural abundance | 20 mM | |
9 | Hepes | natural abundance | 10 mM |