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BMRB: 27207

Gcn4 tAD 1-134

Authors

Tuttle, L.M., Pacheco, D., Warfield, L., Klevit, R.E., Hahn, S.

Assembly

Gcn4 tAD

Entity

1. Gcn4 tAD (polymer, Thiol state: all free), 134 monomers, 14771.04 Da Detail

MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW TSLFDNDIPV TTDD

Formula weight

14771.04 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 43.6 %, Completeness (bb): 80.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.6 % (662 of 1519)	20.1 % (157 of 783)	64.1 % (386 of 602)	88.8 % (119 of 134)
Backbone	80.0 % (624 of 780)	49.4 % (128 of 259)	94.5 % (377 of 399)	97.5 % (119 of 122)
Sidechain	19.1 % (166 of 870)	5.7 % (30 of 524)	40.7 % (136 of 334)	0.0 % (0 of 12)
Aromatic	3.1 % (4 of 128)	6.3 % (4 of 64)	0.0 % (0 of 63)	0.0 % (0 of 1)
Methyl	13.1 % (17 of 130)	6.2 % (4 of 65)	20.0 % (13 of 65)

1. Gcn4 tAD

MSEYQPSLFA LNPMGFSPLD GSKSTNENVS ASTSTAKPMV GQLIFDKFIK TEEDPIIKQD TPSNLDFDFA LPQTATAPDA KTVLPIPELD DAVVESFFSS STDSTPMFEY ENLEDNSKEW TSLFDNDIPV TTDD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Gcn4 tAD	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	DTT	natural abundance		5 mM
5	PMSF	natural abundance		0.1 mM
6	EDTA	natural abundance		0.1 mM

LACS Plot; CA

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: 0.32 ppm, Outliers: 1 Detail

Release date

2017-08-06

Citation

Gcn4-Mediator Specificity Is Mediated by a Large and Dynamic Fuzzy Protein-Protein Complex
Tuttle, L.M., Pacheco, D., Warfield, L., Luo, J.M., Ranish, J., Hahn, S.M., Klevit, R.E.
Cell Rep. (2018), 22, 3251-3264, PubMed 29562181 , DOI 10.1016/j.celrep.2018.02.097 , Abstract

Related entities 1. Gcn4 tAD, : 2 entities Detail

Interaction partners 1. Gcn4 tAD, : 13 interactors Detail

More details for 13 interactors identified by 9 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Gcn4 tAD	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	DTT	natural abundance		5 mM
5	PMSF	natural abundance		0.1 mM
6	EDTA	natural abundance		0.1 mM

2 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Gcn4 tAD	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	DTT	natural abundance		5 mM
5	PMSF	natural abundance		0.1 mM
6	EDTA	natural abundance		0.1 mM

3 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Gcn4 tAD	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	DTT	natural abundance		5 mM
5	PMSF	natural abundance		0.1 mM
6	EDTA	natural abundance		0.1 mM

4 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Gcn4 tAD	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	DTT	natural abundance		5 mM
5	PMSF	natural abundance		0.1 mM
6	EDTA	natural abundance		0.1 mM

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Gcn4 tAD	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	DTT	natural abundance		5 mM
5	PMSF	natural abundance		0.1 mM
6	EDTA	natural abundance		0.1 mM

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Gcn4 tAD	[U-100% 13C; U-100% 15N]		500 uM
2	sodium phosphate	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM
4	DTT	natural abundance		5 mM
5	PMSF	natural abundance		0.1 mM
6	EDTA	natural abundance		0.1 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr27207_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr27207_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	614		98.5 (chain: 1, length: 134)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 134, Sequence coverage: 98.5%
- Total number of assignments
  - ¹³C chemical shifts: 376
  - ¹H chemical shifts: 119
  - ¹⁵N chemical shifts: 119
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords Gcn4, Med15, fuzzy binding, transcription