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BMRB: 27211

6F3K

Solid-state NMR assignment of P. horikoshii TET2

Authors

Sivertsen, A.C., Schanda, P.

Assembly

TET

Entity

1. TET (polymer, Thiol state: all free), 353 monomers, 39013.56 Da Detail

MEVRNMVDYE LLKKVVEAPG VSGYEFLGIR DVVIEEIKDY VDEVKVDKLG NVIAHKKGEG PKVMIAAHMD QIGLMVTHIE KNGFLRVAPI GGVDPKTLIA QRFKVWIDKG KFIYGVGASV PPHIQKPEDR KKAPDWDQIF IDIGAESKEE AEDMGVKIGT VITWDGRLER LGKHRFVSIA FDDRIAVYTI LEVAKQLKDA KADVYFVATV QEEVGLRGAR TSAFGIEPDY GFAIDVTIAA DIPGTPEHKQ VTHLGKGTAI KIMDRSVICH PTIVRWLEEL AKKHEIPYQL EILLGGGTDA GAIHLTKAGV PTGALSVPAR YIHSNTEVVD ERDVDATVEL MTKALENIHE LKI

Formula weight

39013.56 Da

Source organism

Pyrococcus horikoshii

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.1 %, Completeness: 42.0 %, Completeness (bb): 64.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	42.0 % (1720 of 4096)	15.3 % (324 of 2117)	68.2 % (1107 of 1624)	81.4 % (289 of 355)
Backbone	64.4 % (1344 of 2088)	30.1 % (217 of 722)	81.6 % (839 of 1028)	85.2 % (288 of 338)
Sidechain	27.0 % (629 of 2330)	7.7 % (107 of 1395)	56.8 % (521 of 918)	5.9 % (1 of 17)
Aromatic	0.0 % (0 of 268)	0.0 % (0 of 134)	0.0 % (0 of 130)	0.0 % (0 of 4)
Methyl	40.5 % (191 of 472)	5.9 % (14 of 236)	75.0 % (177 of 236)

1. TET

Show sample condition

Sample #1

Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

Sample #2

Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 2H,13C,15N labelled

#	Name	Isotope labeling	Concentration
4	TRIS	natural abundance	50 (±5.0) mM
5	sodium chloride	natural abundance	20 (±1.0) mM
6	TET	[U-13C; U-15N; U-2H]	50 (±10.0) % w/w

Sample #3

Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD

#	Name	Isotope labeling	Concentration
7	TRIS	natural abundance	50 (±5.0) mM
8	sodium chloride	natural abundance	20 (±1.0) mM
9	TET	13C,15N at Ile, Leu, Val	50 (±10.0) % w/w

Sample #4

Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 13C,15N at Leu, Lys, Pro

#	Name	Isotope labeling	Concentration
10	TRIS	natural abundance	50 (±5.0) mM
11	sodium chloride	natural abundance	20 (±1.0) mM
12	TET	13C,15N at Leu, Lys, Pro	50 (±10.0) % w/w

Sample #5

Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 13C,15N at Gly, Tyr, Phe, Arg

#	Name	Isotope labeling	Concentration
13	TRIS	natural abundance	50 (±5.0) mM
14	sodium chloride	natural abundance	20 (±1.0) mM
15	TET	13C,15N at Gly, Tyr, Phe, Arg	50 (±10.0) % w/w

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	adamantane	methyl carbon	31.47 ppm	external	direct	0.2514495
¹H	DSS	methyl carbon	0.0 ppm	internal	direct	1.0
¹⁵N	na	na	0.0 na	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	adamantane	methyl carbon	31.47 ppm	external	direct	0.2514495
¹H	DSS	methyl carbon	0.0 ppm	internal	direct	1.0
¹⁵N	na	na	0.0 na	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	adamantane	methyl carbon	31.47 ppm	external	direct	0.2514495
¹H	DSS	methyl carbon	0.0 ppm	internal	direct	1.0
¹⁵N	na	na	0.0 na	na	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 6F3K, Strand ID: A Detail

Release date

2017-08-08

Citation

Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex
Gauto, D.F., Estrozi, L.F., Schwieters, C.D., Effantin, G., Macek, P., Sounier, R., Sivertsen, A.C., Schmidt, E., Kerfah, R., Mas, G., Colletier, J.P., Guntert, P., Favier, A., Schoehn, G., Schanda, P., Boisbouvier, J.
Nat. Commun. (2019), 10, 2697-2697, PubMed 31217444 , DOI 10.1038/s41467-019-10490-9 , Abstract

Related entities 1. TET, : 1 : 6 : 43 entities Detail

Experiments performed 36 experiments Detail

1 CCdream (CC)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

2 CANCO (hCANCO)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

3 Expt_3 (hCA[N])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

4 NCO (hNCO)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

5 CC_ILV (CC)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD

#	Name	Isotope labeling	Concentration
7	TRIS	natural abundance	50 (±5.0) mM
8	sodium chloride	natural abundance	20 (±1.0) mM
9	TET	13C,15N at Ile, Leu, Val	50 (±10.0) % w/w

6 ILV_NCA (hCA[N])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

7 ILV_coNCA (hCA[N])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD

#	Name	Isotope labeling	Concentration
7	TRIS	natural abundance	50 (±5.0) mM
8	sodium chloride	natural abundance	20 (±1.0) mM
9	TET	13C,15N at Ile, Leu, Val	50 (±10.0) % w/w

8 1D_13C (C)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

9 HCC (H[C[C]])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

10 NCA_on (hCA[N])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

11 NCOCX (NCOCA)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

12 ILV_NCO (hNCO)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD

#	Name	Isotope labeling	Concentration
7	TRIS	natural abundance	50 (±5.0) mM
8	sodium chloride	natural abundance	20 (±1.0) mM
9	TET	13C,15N at Ile, Leu, Val	50 (±10.0) % w/w

13 CANcoCX (ca_NCO_C.onebond,relayed)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

14 NCO_1000MHz (hNCO)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

15 NCA_1000MHz (hCA[N])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

16 NCACX_1000MHz (N_CA_C.onebond,relayed)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

17 NCACB_Lp (N_CA_CB.onebond,relayed-alternate)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

18 4D_CANCOCX (CA_NCO_C.onebond,relayed)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

19 DARR_600MHz (C_C.through-space)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

20 ILV_NCACX (NCO_C.relayed)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD

#	Name	Isotope labeling	Concentration
7	TRIS	natural abundance	50 (±5.0) mM
8	sodium chloride	natural abundance	20 (±1.0) mM
9	TET	13C,15N at Ile, Leu, Val	50 (±10.0) % w/w

21 NCOCX_GHz (NCO_C.relayed)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

22 ILV_NCOCX (NCO_C.relayed)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD

#	Name	Isotope labeling	Concentration
7	TRIS	natural abundance	50 (±5.0) mM
8	sodium chloride	natural abundance	20 (±1.0) mM
9	TET	13C,15N at Ile, Leu, Val	50 (±10.0) % w/w

23 4D_CB

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

24 NCA_longCP (hCA[N])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

25 NCO_longCP (CO[N])

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

26 NCACX_longCP (N_CA_C.onebond,relayed)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD, 13C,15N labelled

#	Name	Isotope labeling	Concentration
1	TRIS	natural abundance	50 (±5.0) mM
2	sodium chloride	natural abundance	20 (±1.0) mM
3	TET	[U-99% 13C; U-99% 15N]	50 (±10.0) % w/w

27 NCA_LKP (N_CA.onebond)

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 13C,15N at Leu, Lys, Pro

#	Name	Isotope labeling	Concentration
10	TRIS	natural abundance	50 (±5.0) mM
11	sodium chloride	natural abundance	20 (±1.0) mM
12	TET	13C,15N at Leu, Lys, Pro	50 (±10.0) % w/w

28 2D 1H-15N HSQC/HMQC

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 2H,13C,15N labelled

#	Name	Isotope labeling	Concentration
4	TRIS	natural abundance	50 (±5.0) mM
5	sodium chloride	natural abundance	20 (±1.0) mM
6	TET	[U-13C; U-15N; U-2H]	50 (±10.0) % w/w

29 3D hCANH

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 2H,13C,15N labelled

#	Name	Isotope labeling	Concentration
4	TRIS	natural abundance	50 (±5.0) mM
5	sodium chloride	natural abundance	20 (±1.0) mM
6	TET	[U-13C; U-15N; U-2H]	50 (±10.0) % w/w

30 3D hCONH

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 2H,13C,15N labelled

#	Name	Isotope labeling	Concentration
4	TRIS	natural abundance	50 (±5.0) mM
5	sodium chloride	natural abundance	20 (±1.0) mM
6	TET	[U-13C; U-15N; U-2H]	50 (±10.0) % w/w

31 3D hcaCBcaNH

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 2H,13C,15N labelled

#	Name	Isotope labeling	Concentration
4	TRIS	natural abundance	50 (±5.0) mM
5	sodium chloride	natural abundance	20 (±1.0) mM
6	TET	[U-13C; U-15N; U-2H]	50 (±10.0) % w/w

32 3D hcoCAcoNH

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 2H,13C,15N labelled

#	Name	Isotope labeling	Concentration
4	TRIS	natural abundance	50 (±5.0) mM
5	sodium chloride	natural abundance	20 (±1.0) mM
6	TET	[U-13C; U-15N; U-2H]	50 (±10.0) % w/w

33 coNCA_LKP

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 13C,15N at Leu, Lys, Pro

#	Name	Isotope labeling	Concentration
10	TRIS	natural abundance	50 (±5.0) mM
11	sodium chloride	natural abundance	20 (±1.0) mM
12	TET	13C,15N at Leu, Lys, Pro	50 (±10.0) % w/w

34 LKP_NCAshort

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 13C,15N at Leu, Lys, Pro

#	Name	Isotope labeling	Concentration
10	TRIS	natural abundance	50 (±5.0) mM
11	sodium chloride	natural abundance	20 (±1.0) mM
12	TET	13C,15N at Leu, Lys, Pro	50 (±10.0) % w/w

35 NCA

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 13C,15N at Gly, Tyr, Phe, Arg

#	Name	Isotope labeling	Concentration
13	TRIS	natural abundance	50 (±5.0) mM
14	sodium chloride	natural abundance	20 (±1.0) mM
15	TET	13C,15N at Gly, Tyr, Phe, Arg	50 (±10.0) % w/w

36 coNCA

Instrument

Varian UnityPlus - 600 MHz

Sample

State isotropic, Solvent system H2O, Pressure 1 atm, Temperature 298 (±2) K, pH 7.5 (±0.1), Details TET2_MPD_DCN, 13C,15N at Gly, Tyr, Phe, Arg

#	Name	Isotope labeling	Concentration
13	TRIS	natural abundance	50 (±5.0) mM
14	sodium chloride	natural abundance	20 (±1.0) mM
15	TET	13C,15N at Gly, Tyr, Phe, Arg	50 (±10.0) % w/w

nullKeywords aminopeptidase, protein quality control machinery

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Found 1 Document (1.15 seconds)

BMRB: 27211

Sample #1

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. TET, : 1 : 6 : 43 entities Detail

Experiments performed 36 experiments Detail

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