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BMRB: 27240

5Z55

NMR backbone and side chain assignments of the Kringle domain of ROR1

Authors

Ma, X., Hu, K.

Assembly

ROR1 Kringle monomer

Entity

1. ROR1 Kringle monomer (polymer, Thiol state: all disulfide bound), 85 monomers, 9550.422 Da Detail

GSKCYNSTGV DYRGTVSVTK SGRQCQPWNS QYPHTHTFTA LRFPELNGGH SYCRNPGNQK EAPWCFTLDE NFKSDLCDIP ACDSK

Formula weight

9550.422 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.5 %, Completeness: 91.7 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.7 % (885 of 965)	93.1 % (469 of 504)	89.7 % (332 of 370)	92.3 % (84 of 91)
Backbone	92.0 % (458 of 498)	94.7 % (162 of 171)	90.3 % (224 of 248)	91.1 % (72 of 79)
Sidechain	91.9 % (501 of 545)	92.2 % (307 of 333)	91.0 % (182 of 200)	100.0 % (12 of 12)
Aromatic	85.2 % (92 of 108)	85.2 % (46 of 54)	84.6 % (44 of 52)	100.0 % (2 of 2)
Methyl	100.0 % (52 of 52)	100.0 % (26 of 26)	100.0 % (26 of 26)

1. Kringle domain of ROR1

GSKCYNSTGV DYRGTVSVTK SGRQCQPWNS QYPHTHTFTA LRFPELNGGH SYCRNPGNQK EAPWCFTLDE NFKSDLCDIP ACDSK

Show sample condition

Sample

Solvent system 94% H2O/6% D2O, Temperature 298 K, pH 8.0

#	Name	Isotope labeling	Type	Concentration
1	Kringle domain of ROR1	[U-99% 13C; U-99% 15N]		0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.38 ppm, Outliers: 1 Detail

Release date

2017-08-29

Citation

Backbone and side-chain chemical shift assignments of the kringle domain of human receptor tyrosine kinase-like orphan receptor 1 (ROR1)
Ma, X., Zhang, Y., Liu, B., Yang, J., Hu, K.
Biomol. NMR Assign. (2018), 12, 145-148, PubMed 29313214 , DOI 10.1007/s12104-017-9797-9 , Abstract

Related entities 1. ROR1 Kringle monomer, : 1 : 5 : 438 entities Detail

Interaction partners 1. ROR1 Kringle monomer, : 9 interactors Detail

More details for 9 interactors identified by 5 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC