BMRB: 27242

Trigger Factor

Authors

Morgado, L., Burmann, B., Hiller, S.

Assembly

Trigger factor

Entity

1. Trigger factor (polymer, Thiol state: not present), 432 monomers, 48192.05 Da Detail

MQVSVETTQG LGRRVTITIA ADSIETAVKS ELVNVAKKVR IDGFRKGKVP MNIVAQRYGA SVRQDVLGDL MSRNFIDAII KEKINPAGAP TYVPGEYKLG EDFTYSVEFE VYPEVELQGL EAIEVEKPIV EVTDADVDGM LDTLRKQQAT WKEKDGAVEA EDRVTIDFTG SVDGEEFEGG KASDFVLAMG QGRMIPGFED GIKGHKAGEE FTIDVTFPEE YHAENLKGKA AKFAINLKKV EERELPELTA EFIKRFGVED GSVEGLRAEV RKNMERELKS AIRNRVKSQA IEGLVKANDI DVPAALIDSE IDVLRRQAAQ RFGGNEKQAL ELPRELFEEQ AKRRVVVGLL LGEVIRTNEL KADEERVKGL IEEMASAYED PKEVIEFYSK NKELMDNMRN VALEEQAVEA VLAKAKVTEK ETTFNELMNQ QA

Formula weight

48192.05 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 53.0 %, Completeness: 18.8 %, Completeness (bb): 29.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	18.8 % (939 of 5007)	15.0 % (393 of 2615)	16.9 % (327 of 1939)	48.3 % (219 of 453)
Backbone	29.6 % (760 of 2568)	30.0 % (265 of 884)	22.0 % (278 of 1264)	51.7 % (217 of 420)
Sidechain	10.5 % (297 of 2839)	7.5 % (129 of 1731)	15.4 % (166 of 1075)	6.1 % (2 of 33)
Aromatic	6.3 % (16 of 254)	6.3 % (8 of 127)	5.6 % (7 of 126)	100.0 % (1 of 1)
Methyl	9.4 % (48 of 512)	7.8 % (20 of 256)	10.9 % (28 of 256)

1. TF

MQVSVETTQG LGRRVTITIA ADSIETAVKS ELVNVAKKVR IDGFRKGKVP MNIVAQRYGA SVRQDVLGDL MSRNFIDAII KEKINPAGAP TYVPGEYKLG EDFTYSVEFE VYPEVELQGL EAIEVEKPIV EVTDADVDGM LDTLRKQQAT WKEKDGAVEA EDRVTIDFTG SVDGEEFEGG KASDFVLAMG QGRMIPGFED GIKGHKAGEE FTIDVTFPEE YHAENLKGKA AKFAINLKKV EERELPELTA EFIKRFGVED GSVEGLRAEV RKNMERELKS AIRNRVKSQA IEGLVKANDI DVPAALIDSE IDVLRRQAAQ RFGGNEKQAL ELPRELFEEQ AKRRVVVGLL LGEVIRTNEL KADEERVKGL IEEMASAYED PKEVIEFYSK NKELMDNMRN VALEEQAVEA VLAKAKVTEK ETTFNELMNQ QA

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	TF	[U-13C; U-15N; U-2H]		1 mM
2	potassium phosphate	natural abundance		20 mM
3	potassium chloride	natural abundance		100 mM
4	EDTA	natural abundance		0.5 mM

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LACS Plot; CA

Referencing offset: 1.03 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 1.03 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 5OWI, Strand ID: A, B Detail

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Release date

2017-08-31

Citation

The dynamic dimer structure of the chaperone Trigger Factor
Morgado, L., Burmann, B.M., Sharpe, T., Mazur, A., Hiller, S.
Nat. Commun. (2017), 8, 1992-1992, PubMed 29222465 , DOI 10.1038/s41467-017-02196-7 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Trigger factor, : 1 : 10 : 1 : 7 : 98 entities Detail

Interaction partners 1. Trigger factor, : 44 interactors Detail

Experiments performed 2 experiments Detail