BMRB: 27289

Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR and Molecular Dynamics Approach

Authors

Keane, S.C., Summers, M.F.

Assembly

DIS

Entity

1. DIS (polymer, Thiol state: not present), 47 monomers, 15422.21 Da Detail

GGCAGGACUC GGCUUGCUGA AGCGCGCACG GCAAGAGGCG AGGGGCC

Formula weight

15422.21 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 52.7 %, Completeness (bb): 50.0 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	52.7 % (214 of 406)	52.7 % (214 of 406)
Suger, PO4	50.0 % (141 of 282)	50.0 % (141 of 282)
Nucleobase	58.9 % (73 of 124)	58.9 % (73 of 124)
Aromatic	74.5 % (73 of 98)	74.5 % (73 of 98)

1. DIS

GGCAGGACUC GGCUUGCUGA AGCGCGCACG GCAAGAGGCG AGGGGCC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	DIS	natural abundance		300 uM
2	TRIS	[U-2H]		20 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	DIS	A2rGr		300 uM
4	TRIS	[U-2H]		20 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	DIS	A28GH		300 uM
6	TRIS	[U-2H]		20 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
7	DIS	G8C6r		300 uM
8	TRIS	[U-2H]		20 mM

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Release date

2017-10-23

Citation

Structure of the 30 kDa HIV-1 RNA Dimerization Signal by a Hybrid Cryo-EM, NMR, and Molecular Dynamics Approach
Zhang, K., Keane, S.C., Zhaoming, S., Irobalieva, R.N., Chen, M., Van, V., Sciandra, C.A., Marchant, J., Heng, X., Schmid, M.F., Case, D.A., Ludtke, S.J., Summers, M.F., Chiu, W.
Structure (2018), S0969-2126, 30001-30007, PubMed 29398526 , DOI 10.1016/j.str.2018.01.001 , Abstract

Related entities 1. DIS, : 1 : 2 entities Detail

Experiments performed 4 experiments Detail