HusA from porphyromonas gingivalis
MGQGTAYAEV MNRKVAALDS VPPTEYATLA ADFSRIAAVE GSDWMAAYYT AYCRIIPAFG NPSEADRLCE EAESMLSKAE SLGGDLSEIA CLRSMAASAR LLVNPQERWQ TYGAESSRQL AVALEANPAN PRAYFLQAQS LLYTPAQFGG GKDKALPFAE KSVSCYAAAT VSPAYAPHWG EQQARQLLML CKAETQELVP R
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.3 % (1970 of 2230) | 96.1 % (1105 of 1150) | 76.3 % (665 of 872) | 96.2 % (200 of 208) |
Backbone | 83.9 % (992 of 1182) | 96.5 % (387 of 401) | 71.5 % (423 of 592) | 96.3 % (182 of 189) |
Sidechain | 93.9 % (1163 of 1238) | 95.9 % (718 of 749) | 90.9 % (427 of 470) | 94.7 % (18 of 19) |
Aromatic | 80.6 % (137 of 170) | 98.8 % (84 of 85) | 61.0 % (50 of 82) | 100.0 % (3 of 3) |
Methyl | 98.6 % (215 of 218) | 98.2 % (107 of 109) | 99.1 % (108 of 109) |
1. HusA
MGQGTAYAEV MNRKVAALDS VPPTEYATLA ADFSRIAAVE GSDWMAAYYT AYCRIIPAFG NPSEADRLCE EAESMLSKAE SLGGDLSEIA CLRSMAASAR LLVNPQERWQ TYGAESSRQL AVALEANPAN PRAYFLQAQS LLYTPAQFGG GKDKALPFAE KSVSCYAAAT VSPAYAPHWG EQQARQLLML CKAETQELVP RSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | HusA | [U-13C; U-15N] | 0.9 mM | |
2 | sodium phosphate | natural abundance | 10 mM | |
3 | DSS | natural abundance | 20 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | HusA | [U-13C; U-15N] | 0.9 mM | |
5 | sodium phosphate | natural abundance | 10 mM | |
6 | DSS | natural abundance | 20 uM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | HusA | natural abundance | 0.9 mM | |
8 | sodium phosphate | natural abundance | 10 mM | |
9 | DSS | natural abundance | 20 uM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | HusA | natural abundance | 0.9 mM | |
11 | sodium phosphate | natural abundance | 10 mM | |
12 | DSS | natural abundance | 20 uM |