1H, 13C, and 15N Chemical Shift Assignments for ubiquitin in sf9 cells by in-cell NMR spectroscopy
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 56.0 % (499 of 891) | 66.0 % (309 of 468) | 34.8 % (119 of 342) | 87.7 % (71 of 81) |
Backbone | 64.7 % (291 of 450) | 94.2 % (146 of 155) | 33.3 % (74 of 222) | 97.3 % (71 of 73) |
Sidechain | 42.3 % (216 of 511) | 52.1 % (163 of 313) | 27.9 % (53 of 190) | 0.0 % (0 of 8) |
Aromatic | 56.3 % (18 of 32) | 75.0 % (12 of 16) | 37.5 % (6 of 16) | |
Methyl | 92.4 % (85 of 92) | 93.5 % (43 of 46) | 91.3 % (42 of 46) |
1. ubiquitin3A
MQIFVKTATG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLAFAGKQL EDGRTLSDYN IQKESTLHLA LRLRGGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 300 K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ubiquitin3A | [U-100% 15N] | 50 (±12.0) uM | |
2 | ubiquitin3A | [U-100% 13C; U-100% 15N] | 50 (±12.0) uM |