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The 15N, 13C and 1H Chemical Shift Assignment of Sis1 J domain from S. cerevisiae

Authors

Pinheiro, G.MS., Amorim, G.C., Iqbal, A., Ramos, C.HI., Almeida, F.CL.

Assembly

Sis1 J-domain

Entity

1. Sis1 J-domain (polymer, Thiol state: not present), 84 monomers, 9366.357 Da Detail

GMTSVKETKL YDLLGVSPSA NEQELKKGYR KAALKYHPDK PTGDTEKFKE ISEAFEILND PQKREIYDQY GLEAARSGGP SFGP

Formula weight

9366.357 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.2 %, Completeness: 83.9 %, Completeness (bb): 91.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.9 % (826 of 985)	81.2 % (423 of 521)	86.6 % (330 of 381)	88.0 % (73 of 83)
Backbone	91.3 % (449 of 492)	87.1 % (148 of 170)	94.3 % (230 of 244)	91.0 % (71 of 78)
Sidechain	79.1 % (450 of 569)	78.3 % (275 of 351)	81.2 % (173 of 213)	40.0 % (2 of 5)
Aromatic	59.5 % (44 of 74)	70.3 % (26 of 37)	48.6 % (18 of 37)
Methyl	89.7 % (61 of 68)	88.2 % (30 of 34)	91.2 % (31 of 34)

1. Sis1 J-domain

GMTSVKETKL YDLLGVSPSA NEQELKKGYR KAALKYHPDK PTGDTEKFKE ISEAFEILND PQKREIYDQY GLEAARSGGP SFGP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.5, Details 250 uM Sis1 J-domain in 25 mM Tris-HCl pH 7.5, 200 mM NaCl and 10% D2O

#	Name	Isotope labeling	Concentration
1	Sis1 J-domain		250 uM
2	TRIS HCl	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.48 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.48 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.33 ppm, Outliers: 2 Detail

Release date

2018-02-14

Citation

¹H, ¹⁵N and ¹³C resonance assignments of the J-domain of co-chaperone Sis1 from Saccharomyces cerevisiae
Pinheiro, G.MS., Amorim, G.C., Iqbal, A., Ramos, C.HI., Almeida, F.CL.
Biomol. NMR Assign. (2018), 12, 279-281, PubMed 29713947 , DOI 10.1007/s12104-018-9823-6 , Abstract

Related entities 1. Sis1 J-domain, : 1 : 4 : 127 entities Detail

Interaction partners 1. Sis1 J-domain, : 554 interactors Detail

More details for 554 interactors identified by 17 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC/HMQC