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BMRB: 27442

5ZMR

Backbone 1H, 13C, and 15N Chemical Shift Assignments for residues 1-136 of yeast Rpn5.

Authors

Zhang, W., Zhao, C., Hu, Y., Jin, C.

Assembly

Rpn5

Entity

1. Rpn5 (polymer, Thiol state: all free), 136 monomers, 15594.83 Da Detail

MSRDAPIKAD KDYSQILKEE FPKIDSLAQN DCNSALDQLL VLEKKTRQAS DLASSKEVLA KIVDLLASRN KWDDLNEQLT LLSKKHGQLK LSIQYMIQKV MEYLKSSKSL DLNTRISVIE TIRVVTENKI FVEVER

Formula weight

15594.83 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 87.0 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.0 % (1434 of 1649)	83.5 % (723 of 866)	89.4 % (567 of 634)	96.6 % (144 of 149)
Backbone	98.0 % (796 of 812)	98.2 % (266 of 271)	97.8 % (398 of 407)	98.5 % (132 of 134)
Sidechain	79.2 % (770 of 972)	76.8 % (457 of 595)	83.1 % (301 of 362)	80.0 % (12 of 15)
Aromatic	18.3 % (11 of 60)	20.0 % (6 of 30)	13.8 % (4 of 29)	100.0 % (1 of 1)
Methyl	86.5 % (154 of 178)	86.5 % (77 of 89)	86.5 % (77 of 89)

1. Rpn5

MSRDAPIKAD KDYSQILKEE FPKIDSLAQN DCNSALDQLL VLEKKTRQAS DLASSKEVLA KIVDLLASRN KWDDLNEQLT LLSKKHGQLK LSIQYMIQKV MEYLKSSKSL DLNTRISVIE TIRVVTENKI FVEVER

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	Rpn5	[U-95% 13C; U-95% 15N]	0.3 mM
2	D2O	[U-99% 2H]	10 % v/v
3	DSS	natural abundance	0.1 mg/mL
4	DTT	natural abundance	1 mM
5	sodium phosphate	natural abundance	50 mM
6	sodium sulfate	natural abundance	50 mM
7	trifluoroethanol	natural abundance	2 % v/v
8	sucrose	natural abundance	5 % w/v

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Release date

2018-04-03

Citation

Solution structure of the N-terminal domain of proteasome lid subunit Rpn5
Zhang, W., Zhao, C., Hu, Y., Jin, C.
Biochem. Biophys. Res. Commun. (2018), 504, 225-230, PubMed 30177392 , DOI 10.1016/j.bbrc.2018.08.159 , Abstract

Related entities 1. Rpn5, : 1 : 21 : 43 entities Detail

Interaction partners 1. Rpn5, : 69 interactors Detail

More details for 69 interactors identified by 10 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC