Chemical shift assignments of the C-terminal domain of human FAT10
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 3.9 % (38 of 971) | 3.6 % (18 of 506) | 4.5 % (17 of 377) | 3.4 % (3 of 88) |
Backbone | 5.1 % (26 of 506) | 4.5 % (8 of 178) | 6.1 % (15 of 245) | 3.6 % (3 of 83) |
Sidechain | 3.1 % (17 of 540) | 3.0 % (10 of 328) | 3.4 % (7 of 207) | 0.0 % (0 of 5) |
Aromatic | 0.0 % (0 of 40) | 0.0 % (0 of 20) | 0.0 % (0 of 20) | |
Methyl | 0.0 % (0 of 104) | 0.0 % (0 of 52) | 0.0 % (0 of 52) |
1. fat10 c
GAMGDEELPL FLVESGDEAK RHLLQVRRSS SVAQVKAMIE TKTGIIPETQ IVTLNGKRLE DGKMMADYGI RKGNLLFLAS YSIGGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | fat10_c | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | sodium chloride | natural abundance | 150 mM | |
3 | sodium phosphate | natural abundance | 20 mM |