Backbone 1H, 13C, and 15N Chemical Shift Assignments for hFABP1 triple-mutant (K57A,E77A,K96A)
GSMSFSGKYQ LQSQENFEAF MKAIGLPEEL IQKGKDIKGV SEIVQNGKHF KFTITAGSAV IQNEFTVGEE CELETMTGAK VKTVVQLEGD NKLVTTFANI KSVTELNGDI ITNTMTLGDI VFKRISKRI
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 34.2 % (516 of 1510) | 18.0 % (141 of 783) | 43.1 % (252 of 585) | 86.6 % (123 of 142) |
Backbone | 64.2 % (496 of 772) | 47.2 % (127 of 269) | 65.6 % (246 of 375) | 96.1 % (123 of 128) |
Sidechain | 15.8 % (135 of 855) | 2.7 % (14 of 514) | 37.0 % (121 of 327) | 0.0 % (0 of 14) |
Aromatic | 0.0 % (0 of 92) | 0.0 % (0 of 46) | 0.0 % (0 of 46) | |
Methyl | 7.1 % (11 of 156) | 3.8 % (3 of 78) | 10.3 % (8 of 78) |
1. Human liver fatty acid binding protein (hFABP1) K57A E77A K96A mutant
GSMSFSGKYQ LQSQENFEAF MKAIGLPEEL IQKGKDIKGV SEIVQNGKHF KFTITAGSAV IQNEFTVGEE CELETMTGAK VKTVVQLEGD NKLVTTFANI KSVTELNGDI ITNTMTLGDI VFKRISKRISolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human liver fatty acid binding protein (hFABP1) K57A, E77A, K96A mutant | [U-99% 13C; U-99% 15N] | 0.290 mM | |
2 | MES | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 50 mM | |
4 | sodium azide | natural abundance | 0.02 % | |
5 | D2O | [U-100% 2H] | 10 % | |
6 | H2O | natural abundance | 90 % |