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Found 1 Document (0.507 seconds)

BMRB: 27534

7K3S

Solution NMR Structure of PALB2 Colied-coil Domain (PALB2cc)

Authors: Li, M., Liu, G., Song, F., Swapna, G., Xia, B., Bunting, S.F., Montelione, G.T.
Assembly: PALB2cc
Entity: 1. PALB2cc (polymer, Thiol state: not present), 68 monomers, 7925.989 × 2 Da Detail
MEELSGKPLS YAEKEKLKEK LAFLKKEYSR TLARLQRAKR AEKAKNSKKA IEDGVPQPEA LEHHHHHH
Total weight: 15851.978 Da
Max. entity weight: 7925.989 Da
Source organism: Mus musculus
Exptl. method: solution NMR
Release date: 2018-07-05
Citation: Antiparallel Coiled-Coil Interactions Mediate Homodimerization of the DNA Damage Repair Protein, PALB2
Song, F., Li, M., Liu, G., Swapna, G., Daigham, N.S., Xia, B., Montelione, G.T., Bunting, S.F.
Biochemistry (2018), 57, 6581-6591, PubMed 30289697 , DOI 10.1021/acs.biochem.8b00789 , Abstract

Related entities 1. PALB2cc, : 1 : 1 : 2 : 3 entities Detail

Experiments performed 15 experiments Detail

1 2D TROSY-HSQC

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

2 3D TROSY-HNCOCA

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

3 3D TROSY-HNCA

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

4 3D TROSY-HNCOCACB

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

5 3D TROSY-HNCACB

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

6 3D TROSY-HNCO

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

7 3D TROSY-HBHACONH

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

8 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

9 3D CCH-TOCSY

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

10 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

11 AROMATIC CCH-TOCSY

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

12 3D 1H 13C NOESY

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

13 3D simultaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

14 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	PALB2cc	[U-100% 13C; U-100% 15N]	1.2 mM
2	MES	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	calcium chloride	natural abundance	5 mM
5	DTT	natural abundance	10 mM
6	sodium azide	natural abundance	0.02 %
7	H2O	natural abundance	90 %
8	DSS	natural abundance	10 %

15 X-filtered noesy (pulse sequence: noesyhsqcgpwgx13d)

Instrument

Bruker Avance - 800 MHz cryo-probe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details 1:2 sample of 13C,15N enriched to unlabeled (natural abundance) protein. This sample used to identify the dimer interface in PALB2cc using 13C filtered NOESY experiments ( pulse sequence: noesyhsqcgpwgx13d).

#	Name	Isotope labeling	Concentration
9	PALB2cc	[U-100% 13C; U-100% 15N]	1 na
10	MES	natural abundance	20 mM
11	sodium chloride	natural abundance	200 mM
12	calcium chloride	natural abundance	5 mM
13	DTT	natural abundance	10 mM
14	sodium azide	natural abundance	0.02 %
15	H2O	natural abundance	90 %
16	DSS	natural abundance	10 %
17	PALB2cc	natural abundance	2 na

Keywords Antiparallel, Coiled coil, Homologous recombination, PALB2

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Found 1 Document (0.507 seconds)

BMRB: 27534

Related entities 1. PALB2cc, : 1 : 1 : 2 : 3 entities Detail

Experiments performed 15 experiments Detail

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