1H, 15N, and 13C triple resonance assignments of innate immune evasion protein EapH2 from the S. aureus
GSTAEKDKLP ATQKAKEMQN VPYTIAVDGI MAFNQSYLNL PKDSQLSYLD LGNKVKALLY DERGVTPEKI RNAKSAVYTI TWKDGSKKEV DLKKDSYTAN LFDSNSIKQI DINVKTK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 45.3 % (629 of 1390) | 23.6 % (172 of 728) | 64.5 % (345 of 535) | 88.2 % (112 of 127) |
Backbone | 79.8 % (554 of 694) | 51.1 % (120 of 235) | 93.1 % (322 of 346) | 99.1 % (112 of 113) |
Sidechain | 22.5 % (182 of 808) | 10.5 % (52 of 493) | 43.2 % (130 of 301) | 0.0 % (0 of 14) |
Aromatic | 10.0 % (8 of 80) | 12.5 % (5 of 40) | 7.7 % (3 of 39) | 0.0 % (0 of 1) |
Methyl | 16.2 % (21 of 130) | 10.8 % (7 of 65) | 21.5 % (14 of 65) |
1. Extracellular Adherence Protein Homolog 2
GSTAEKDKLP ATQKAKEMQN VPYTIAVDGI MAFNQSYLNL PKDSQLSYLD LGNKVKALLY DERGVTPEKI RNAKSAVYTI TWKDGSKKEV DLKKDSYTAN LFDSNSIKQI DINVKTKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Extracellular Adherence Protein Homolog 2 | [U-99% 13C; U-99% 15N] | 0.75 mM | |
2 | sodium phosphate | natural abundance | 50 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Extracellular Adherence Protein Homolog 2 | [U-99% 15N] | 0.75 mM | |
4 | sodium phosphate | natural abundance | 50 mM |