BMRB: 27547

Backbone chemical shift assignments of H2A in H2A-H2B dimer

Authors

van Ingen, H., Eerland, J., Horn, V., Svatos, A., Zhang, H.

Assembly

H2A/H2B dimer

Entity

1. H2A (polymer, Thiol state: not present), 123 monomers, 13231.32 Da Detail

SGRGKGGKVK GKAKSRSNRA GLQFPVGRIH RLLRKGNYAE RVGAGAPVYL AAVMEYLAAE VLELAGNAAR DNKKTRIIPR HLQLAIRNDE ELNKLLSGVT IAQGGVLPNI QAVLLPKKTE KKA

2. H2B (polymer, Thiol state: not present), 123 monomers, 13677.77 Da Detail

IPPKTSGKAA KKAGKAQKNI TKTDKKKKRK RKESYAIYIY KVLKQVHPDT GISSKAMSIM NSFVNDIFER IAAEASRLAH YNKRSTITSR EIQTAVRLLL PGELAKHAVS EGTKAVTKYT SSK

Total weight

26909.09 Da

Max. entity weight

13677.77 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 50.0 %, Completeness: 22.3 %, Completeness (bb): 40.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	22.3 % (645 of 2891)	11.2 % (171 of 1524)	32.0 % (356 of 1112)	46.3 % (118 of 255)
Backbone	40.5 % (591 of 1458)	25.9 % (130 of 502)	48.0 % (345 of 719)	48.9 % (116 of 237)
Sidechain	9.8 % (163 of 1660)	4.1 % (42 of 1022)	19.2 % (119 of 620)	11.1 % (2 of 18)
Aromatic	0.0 % (0 of 114)	0.0 % (0 of 57)	0.0 % (0 of 57)
Methyl	10.5 % (31 of 294)	6.1 % (9 of 147)	15.0 % (22 of 147)

1. H2A

SGRGKGGKVK GKAKSRSNRA GLQFPVGRIH RLLRKGNYAE RVGAGAPVYL AAVMEYLAAE VLELAGNAAR DNKKTRIIPR HLQLAIRNDE ELNKLLSGVT IAQGGVLPNI QAVLLPKKTE KKA

2. H2B

IPPKTSGKAA KKAGKAQKNI TKTDKKKKRK RKESYAIYIY KVLKQVHPDT GISSKAMSIM NSFVNDIFER IAAEASRLAH YNKRSTITSR EIQTAVRLLL PGELAKHAVS EGTKAVTKYT SSK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	H2A	[U-100% 13C; U-100% 15N; U-80% 2H]		0.3 mM
2	H2B	natural abundance		0.3 mM
3	MES	natural abundance		20 mM
4	sodium chloride	natural abundance		90 mM

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LACS Plot; CA

Referencing offset: 0.47 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.7 ppm	internal	indirect	0.2514495
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	water	protons	4.7 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.47 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.7 ppm	internal	indirect	0.2514495
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	water	protons	4.7 ppm	internal	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.23 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	water	protons	4.7 ppm	internal	indirect	0.2514495
1H	water	protons	4.7 ppm	internal	direct	1.0
15N	water	protons	4.7 ppm	internal	indirect	0.1013291

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Release date

2018-07-16

Citation

Structural basis of specific H2A K13/K15 ubiquitination by RNF168
Horn, V., Uckelmann, M., Zhang, H., Eerland, J., Aarsman, I., Le Paige, U., Davidovich, C., Sixma, T., van Ingen, H.
Nat. Commun. (2019), 10, 1751-1751, PubMed 30988309 , DOI 10.1038/s41467-019-09756-z , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. H2A, : 2 : 1 : 206 entities Detail

Related entities 2. H2B, : 1 : 5 : 4 : 248 entities Detail

Interaction partners 1. H2A, : 12 : 1 interactors Detail

Interaction partners 2. H2B, : 10 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 4 contents Detail