BMRBj - BMREntryMaster

Found 1 Document (3.559 seconds)

BMRB: 27571

5EYO

Backbone 1H, 13C, and 15N Chemical Shift Assignments for the bHLHZip c-MYC:MAX complex.

Authors

Bycroft, M., Zinzalla, G.

Assembly

c-MYC/MAX

Entity

1. c-MYC (polymer, Thiol state: not present), 105 monomers, 12423.00 Da Detail

GSSHHHHHHS SGLVPRGSHM NVKRRTHNVL ERQRRNELKR SFFALRDQIP ELENNEKAPK VVILKKATAY ILSVQAEEQK LISEEDLLRK RREQLKHKLE QLRNS

2. MAX (polymer, Thiol state: not present), 82 monomers, 9754.772 Da Detail

ADKRAHHNAL ERKRRDHIKD SFHSLRDSVP SLQGEKASRA QILDKATEYI QYMRRKNHTH QQDIDDLKRQ NALLEQQVRA LE

Total weight

22177.773 Da

Max. entity weight

12423.0 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 48.1 %, Completeness: 21.4 %, Completeness (bb): 39.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	21.4 % (489 of 2283)	10.2 % (124 of 1219)	32.9 % (282 of 858)	40.3 % (83 of 206)
Backbone	39.2 % (437 of 1114)	25.1 % (94 of 374)	46.7 % (260 of 557)	45.4 % (83 of 183)
Sidechain	10.4 % (141 of 1352)	3.7 % (31 of 845)	22.7 % (110 of 484)	0.0 % (0 of 23)
Aromatic	0.0 % (0 of 114)	0.0 % (0 of 57)	0.0 % (0 of 57)
Methyl	12.1 % (22 of 182)	7.7 % (7 of 91)	16.5 % (15 of 91)

1. c-MYC

GSSHHHHHHS SGLVPRGSHM NVKRRTHNVL ERQRRNELKR SFFALRDQIP ELENNEKAPK VVILKKATAY ILSVQAEEQK LISEEDLLRK RREQLKHKLE QLRNS

2. MAX

ADKRAHHNAL ERKRRDHIKD SFHSLRDSVP SLQGEKASRA QILDKATEYI QYMRRKNHTH QQDIDDLKRQ NALLEQQVRA LE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	c-MYC	[U-100% 13C; U-100% 15N; U-80% 2H]	250 uM
2	MAX	[U-100% 13C; U-100% 15N; U-80% 2H]	250 uM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	150 mM

Chem. Shift Complete2

Sequence coverage: 44.4 %, Completeness: 20.2 %, Completeness (bb): 37.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	20.2 % (922 of 4566)	9.8 % (240 of 2438)	30.4 % (522 of 1716)	38.8 % (160 of 412)
Backbone	37.7 % (839 of 2228)	24.3 % (182 of 748)	44.6 % (497 of 1114)	43.7 % (160 of 366)
Sidechain	9.2 % (250 of 2704)	3.5 % (59 of 1690)	19.7 % (191 of 968)	0.0 % (0 of 46)
Aromatic	0.0 % (0 of 228)	0.0 % (0 of 114)	0.0 % (0 of 114)
Methyl	8.2 % (30 of 364)	4.4 % (8 of 182)	12.1 % (22 of 182)

1. c-MYC

GSSHHHHHHS SGLVPRGSHM NVKRRTHNVL ERQRRNELKR SFFALRDQIP ELENNEKAPK VVILKKATAY ILSVQAEEQK LISEEDLLRK RREQLKHKLE QLRNS

2. MAX

ADKRAHHNAL ERKRRDHIKD SFHSLRDSVP SLQGEKASRA QILDKATEYI QYMRRKNHTH QQDIDDLKRQ NALLEQQVRA LE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	c-MYC	[U-100% 13C; U-100% 15N; U-80% 2H]	250 uM
2	MAX	[U-100% 13C; U-100% 15N; U-80% 2H]	250 uM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	150 mM

Release date

2018-08-05

Citation

The structure of INI1/hSNF5 RPT1 and its interactions with the c-MYC:MAX heterodimer provide insights into the interplay between MYC and the SWI/SNF chromatin remodeling complex
Sammak, S., Hamdani, N., Gorrec, F., Allen, M.D., Freund, S.D., Bycroft, M., Zinzalla, G.
FEBS J. (2018), PubMed 30222246 , DOI 10.1111/febs.14660 , Abstract

Related entities 1. c-MYC, : 63 entities Detail

Related entities 2. MAX, : 1 : 11 : 5 : 111 entities Detail

Interaction partners 1. c-MYC, : 204 : 1 interactors Detail

More details for 205 interactors identified by 41 citations

Interaction partners 2. MAX, : 85 : 1 interactors Detail

More details for 86 interactors identified by 19 citations

Experiments performed 5 experiments Detail

Chemical shift validation 5 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr27571_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr27571_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	403	80.0 (chain: 1, length: 105)
2	Assigned chemical shifts	assigned_chem_shift_list_2	Warning	361	90.2 (chain: 2, length: 82)
1	Chemical shift references	chemical_shift_reference_1	OK	3	No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 105, Sequence coverage: 80.0%
- Total number of assignments
  - ¹H chemical shifts: 78
  - ¹³C chemical shifts: 247
  - ¹⁵N chemical shifts: 78
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: assigned_chem_shift_list_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 2, Chain length: 82, Sequence coverage: 90.2%
- Total number of assignments
  - ¹H chemical shifts: 71
  - ¹³C chemical shifts: 219
  - ¹⁵N chemical shifts: 71
- Completeness of assignments
  - Entity_assemble_ID: 2
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	691	78	11.3
¹³C chemical shifts	488	247	50.6
¹⁵N chemical shifts	126	78	61.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	210	78	37.1
¹³C chemical shifts	210	163	77.6
¹⁵N chemical shifts	102	78	76.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	528	71	13.4
¹³C chemical shifts	370	219	59.2
¹⁵N chemical shifts	102	71	69.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	164	71	43.3
¹³C chemical shifts	164	147	89.6
¹⁵N chemical shifts	81	71	87.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	481	0	0.0
¹³C chemical shifts	278	84	30.2
¹⁵N chemical shifts	24	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	364	0	0.0
¹³C chemical shifts	206	72	35.0
¹⁵N chemical shifts	21	0	0.0

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Found 1 Document (3.559 seconds)

BMRB: 27571

Sample

Sample

Related entities 1. c-MYC, : 63 entities Detail

Related entities 2. MAX, : 1 : 11 : 5 : 111 entities Detail

Interaction partners 1. c-MYC, : 204 : 1 interactors Detail

Interaction partners 2. MAX, : 85 : 1 interactors Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 5 contents Detail