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Resonance assignments for Ras-related C3 botulinum toxin substrate 1 bound to GDP and Mg2+
Authors
Toyama, Y., Kontani, K., Katada, T., Shimada, I.
Assembly
Rac1-Mg-GDP
Entity
1. Ras-related C3 botulinum toxin substrate 1 (polymer, Thiol state: all free), 192 monomers, 21223.37 Da Detail

GPLGSPEFMQ AIKCVVVGDG AVGKTCLLIS YTTNAFPGEY IPTVFDNYSA NVMVDGKPVN LGLWDTAGQE DYDRLRPLSY PQTDVFLICF SLVSPASFEN VRAKWYPEVR HHCPNTPIIL VGTKLDLRDD KDTIEKLKEK KLTPITYPQG LAMAKEIGAV KYLECSALTQ RGLKTVFDEA IRAVLCPPPV KK


2. Rac1-Mg-GDP, entity MG (non-polymer), 24.305 Da
3. Rac1-Mg-GDP, entity GDP (non-polymer), 443.201 Da
Total weight
21690.875 Da
Max. entity weight
21223.37 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 90.6 %, Completeness: 50.3 %, Completeness (bb): 73.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All50.3 % (1128 of 2242)34.0 % (395 of 1163)64.7 % (576 of 890)83.1 % (157 of 189)
Backbone73.8 % (826 of 1120)51.2 % (195 of 381)84.5 % (476 of 563)88.1 % (155 of 176)
Sidechain35.4 % (460 of 1301)25.7 % (201 of 782)50.8 % (257 of 506)15.4 % (2 of 13)
Aromatic 9.6 % (16 of 166)12.0 % (10 of 83) 4.9 % (4 of 81)100.0 % (2 of 2)
Methyl78.4 % (185 of 236)74.6 % (88 of 118)82.2 % (97 of 118)

1. Ras-related C3 botulinum toxin substrate 1

GPLGSPEFMQ AIKCVVVGDG AVGKTCLLIS YTTNAFPGEY IPTVFDNYSA NVMVDGKPVN LGLWDTAGQE DYDRLRPLSY PQTDVFLICF SLVSPASFEN VRAKWYPEVR HHCPNTPIIL VGTKLDLRDD KDTIEKLKEK KLTPITYPQG LAMAKEIGAV KYLECSALTQ RGLKTVFDEA IRAVLCPPPV KK

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 7.0


#NameIsotope labelingTypeConcentration
1Ras-related C3 botulinum toxin substrate 1{U-2H 13C, 15N, Iled1-[13CH3], Leu, Val-[13CH3, 12C2H3]}0.5 mM
2GUANOSINE-5'-DIPHOSPHATEnatural abundance0.5 mM
3MAGNESIUM IONnatural abundance5 mM
4DTTnatural abundance5 mM
5HEPESnatural abundance20 mM
6DSSnatural abundance0.1 mM

LACS Plot; CA
Referencing offset: 0.59 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.59 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.43 ppm, Outliers: 3 Detail
Release date
2018-08-13
Citation
Conformational landscape alternations promote oncogenic activities of Ras-related C3 botulinum toxin substrate 1 as revealed by NMR
Toyama, Y., Kontani, K., Katada, T., Shimada, I.
Sci. Adv. (2019), 5, eaav8945-eaav8945, PubMed 30891502 , DOI 10.1126/sciadv.aav8945 ,
Related entities 1. Ras-related C3 botulinum toxin substrate 1, : 1 : 63 : 235 entities Detail
Interaction partners 1. Ras-related C3 botulinum toxin substrate 1, : 5 interactors Detail
Experiments performed 10 experiments Detail
Chemical shift validation 5 contents Detail