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BMRB: 27577

7SJ4

Resonance assignments for Ras-related C3 botulinum toxin substrate 1 bound to GDP and Mg2+

Authors

Toyama, Y., Kontani, K., Katada, T., Shimada, I.

Assembly

Rac1-Mg-GDP

Entity

1. Ras-related C3 botulinum toxin substrate 1 (polymer, Thiol state: all free), 192 monomers, 21223.37 Da Detail

GPLGSPEFMQ AIKCVVVGDG AVGKTCLLIS YTTNAFPGEY IPTVFDNYSA NVMVDGKPVN LGLWDTAGQE DYDRLRPLSY PQTDVFLICF SLVSPASFEN VRAKWYPEVR HHCPNTPIIL VGTKLDLRDD KDTIEKLKEK KLTPITYPQG LAMAKEIGAV KYLECSALTQ RGLKTVFDEA IRAVLCPPPV KK

2. Rac1-Mg-GDP, entity MG (non-polymer), 24.305 Da
3. Rac1-Mg-GDP, entity GDP (non-polymer), 443.201 Da

Total weight

21690.875 Da

Max. entity weight

21223.37 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.6 %, Completeness: 50.3 %, Completeness (bb): 73.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	50.3 % (1128 of 2242)	34.0 % (395 of 1163)	64.7 % (576 of 890)	83.1 % (157 of 189)
Backbone	73.8 % (826 of 1120)	51.2 % (195 of 381)	84.5 % (476 of 563)	88.1 % (155 of 176)
Sidechain	35.4 % (460 of 1301)	25.7 % (201 of 782)	50.8 % (257 of 506)	15.4 % (2 of 13)
Aromatic	9.6 % (16 of 166)	12.0 % (10 of 83)	4.9 % (4 of 81)	100.0 % (2 of 2)
Methyl	78.4 % (185 of 236)	74.6 % (88 of 118)	82.2 % (97 of 118)

1. Ras-related C3 botulinum toxin substrate 1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 313 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Ras-related C3 botulinum toxin substrate 1	{U-2H 13C, 15N, Iled1-[13CH3], Leu, Val-[13CH3, 12C2H3]}	0.5 mM
2	GUANOSINE-5'-DIPHOSPHATE	natural abundance	0.5 mM
3	MAGNESIUM ION	natural abundance	5 mM
4	DTT	natural abundance	5 mM
5	HEPES	natural abundance	20 mM
6	DSS	natural abundance	0.1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.59 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.59 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 3 Detail

Release date

2018-08-13

Citation

Conformational landscape alternations promote oncogenic activities of Ras-related C3 botulinum toxin substrate 1 as revealed by NMR
Toyama, Y., Kontani, K., Katada, T., Shimada, I.
Sci. Adv. (2019), 5, eaav8945-eaav8945, PubMed 30891502 , DOI 10.1126/sciadv.aav8945 , Abstract

Related entities 1. Ras-related C3 botulinum toxin substrate 1, : 1 : 63 : 235 entities Detail

Interaction partners 1. Ras-related C3 botulinum toxin substrate 1, : 5 interactors Detail

More details for 5 interactors identified by 3 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC