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BMRB: 27578

7QCC

Putative methyltransferase WBSCR27 in apo-form.

Authors

Mariasina, S.S., Polshakov, V.I., Chang, C.

Assembly

assembly 1

Entity

1. assembly 1 (polymer, Thiol state: all free), 240 monomers, 25963.23 Da Detail

GAMAQEEAGR LPQVLARVGT SHGITDLACK LRFYDDWAPE YDQDVAALKY RAPRLAVDCL SRAFRGSPHD ALILDVACGT GLVAVELQAR GFLQVQGVDG SPEMLKQARA RGLYHHLSLC TLGQEPLPDP EGTFDAVIIV GALSEGQVPC SAIPELLRVT KPGGLVCLTT RTNPSNLPYK ETLEATLDSL ERAGVWECLV TQPVDHWELA TSEQETGLGT CANDGFISGI IYLYRKQETV

Formula weight

25963.23 Da

Source organism

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.5 %, Completeness: 54.1 %, Completeness (bb): 80.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.1 % (1443 of 2668)	42.7 % (586 of 1372)	62.3 % (655 of 1052)	82.8 % (202 of 244)
Backbone	80.9 % (1143 of 1412)	68.2 % (333 of 488)	87.5 % (611 of 698)	88.1 % (199 of 226)
Sidechain	32.8 % (483 of 1474)	28.6 % (253 of 884)	39.7 % (227 of 572)	16.7 % (3 of 18)
Aromatic	0.0 % (0 of 162)	0.0 % (0 of 81)	0.0 % (0 of 78)	0.0 % (0 of 3)
Methyl	37.7 % (116 of 308)	42.2 % (65 of 154)	33.1 % (51 of 154)

1. WBSCR27

GAMAQEEAGR LPQVLARVGT SHGITDLACK LRFYDDWAPE YDQDVAALKY RAPRLAVDCL SRAFRGSPHD ALILDVACGT GLVAVELQAR GFLQVQGVDG SPEMLKQARA RGLYHHLSLC TLGQEPLPDP EGTFDAVIIV GALSEGQVPC SAIPELLRVT KPGGLVCLTT RTNPSNLPYK ETLEATLDSL ERAGVWECLV TQPVDHWELA TSEQETGLGT CANDGFISGI IYLYRKQETV

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	WBSCR27 putative methyltransferase	[U-99% 15N]		0.8 mM
7	NaCl	natural abundance		50 mM
8	sodium phosphate buffer (pH 7)	natural abundance		50 mM
9	DTT	natural abundance		10 mM
10	Sodium azide (NaN3)	natural abundance		0.02 %

Release date

2018-08-13

Citation

Williams-Beuren syndrome-related methyltransferase WBSCR27: cofactor binding and cleavage
Mariasina, S.S., Chang, C.F., Petrova, O.A., Efimov, S.V., Klochkov, V.V., Kechko, O.I., Mitkevich, V.A., Sergiev, P.V., Dontsova, O.A., Polshakov, V.I.
FEBS J. (2020), 287, 5375-5393, PubMed 32255258 , DOI 10.1111/febs.15320 , Abstract

Related entities 1. assembly 1, : 1 : 11 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	WBSCR27 putative methyltransferase	[U-99% 15N]		0.8 mM
7	NaCl	natural abundance		50 mM
8	sodium phosphate buffer (pH 7)	natural abundance		50 mM
9	DTT	natural abundance		10 mM
10	Sodium azide (NaN3)	natural abundance		0.02 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

3 3D HNCO

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

4 3D HCACO

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

5 3D HN(CO)CA

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

6 3D HNCA

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

7 3D CBCA(CO)NH

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

8 3D HNCACB

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

9 3D HBHA(CO)NH

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	WBSCR27 putative methyltransferase	[U-99% 15N]		0.8 mM
7	NaCl	natural abundance		50 mM
8	sodium phosphate buffer (pH 7)	natural abundance		50 mM
9	DTT	natural abundance		10 mM
10	Sodium azide (NaN3)	natural abundance		0.02 %

10 3D HNHAHB

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
6	WBSCR27 putative methyltransferase	[U-99% 15N]		0.8 mM
7	NaCl	natural abundance		50 mM
8	sodium phosphate buffer (pH 7)	natural abundance		50 mM
9	DTT	natural abundance		10 mM
10	Sodium azide (NaN3)	natural abundance		0.02 %

11 3D HCCH-TOCSY

Instrument

Bruker Avance - 850 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	WBSCR27 putative methyltransferase	[U-99% 13C; U-99% 15N]		0.4 mM
2	NaCl	natural abundance		50 mM
3	sodium phosphate buffer (pH 7)	natural abundance		50 mM
4	DTT	natural abundance		10 mM
5	Sodium azide (NaN3)	natural abundance		0.02 %

nullKeywords 5'-Methylthioadenosine, NMR assignments, S-Adenosyl-L-homocysteine, S-Adenosyl-L-methionine, Williams-Beuren syndrome, methyltransferase, protein secondary structure