NMR resonance assignments of the peach allergen Pru p 1
GVFTYESEFT SEIPPPRLFK AFVLDADNLV PKIAPQAIKH SEILEGDGGP GTIKKITFGE GSQYGYVKHK IDSIDKENHS YSYTLIEGDA LGDNLEKISY ETKLVASPSG GSIIKSTSHY HTKGDVEIKE EHVKAGKEKA SNLFKLIETY LKGHPDAYN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 86.5 % (1596 of 1846) | 85.7 % (822 of 959) | 85.5 % (623 of 729) | 95.6 % (151 of 158) |
Backbone | 95.6 % (897 of 938) | 94.5 % (307 of 325) | 96.5 % (446 of 462) | 95.4 % (144 of 151) |
Sidechain | 79.8 % (839 of 1052) | 81.2 % (515 of 634) | 77.1 % (317 of 411) | 100.0 % (7 of 7) |
Aromatic | 10.6 % (17 of 160) | 13.8 % (11 of 80) | 7.5 % (6 of 80) | |
Methyl | 89.6 % (147 of 164) | 89.0 % (73 of 82) | 90.2 % (74 of 82) |
1. Pru p 1.0101
GVFTYESEFT SEIPPPRLFK AFVLDADNLV PKIAPQAIKH SEILEGDGGP GTIKKITFGE GSQYGYVKHK IDSIDKENHS YSYTLIEGDA LGDNLEKISY ETKLVASPSG GSIIKSTSHY HTKGDVEIKE EHVKAGKEKA SNLFKLIETY LKGHPDAYNSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Pru p 1.0101 | [U-99% 13C; U-99% 15N] | 0.6 mM | |
2 | sodium phosphate | natural abundance | 20 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Pru p 1.0101 | [U-99% 15N] | 0.5 mM | |
4 | sodium phosphate | natural abundance | 20 mM |