15N, 13C and 1H backbone resonance assignments of FKBP12 from the pathogenic fungi Mucor circinelloides
MGVTVERIAP GDGKNFPKKG DKVTIHYVGT LENGDKFDSS RDRGSPFQCT IGVGQVIKGW DEGVTQLSVG EKARLICTHD YAYGERGYPG LIPPKATLNF EVELIKIN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 63.9 % (790 of 1236) | 55.3 % (356 of 644) | 69.5 % (335 of 482) | 90.0 % (99 of 110) |
Backbone | 95.0 % (604 of 636) | 93.8 % (211 of 225) | 95.1 % (294 of 309) | 97.1 % (99 of 102) |
Sidechain | 39.2 % (272 of 693) | 34.6 % (145 of 419) | 47.7 % (127 of 266) | 0.0 % (0 of 8) |
Aromatic | 0.0 % (0 of 92) | 0.0 % (0 of 46) | 0.0 % (0 of 45) | 0.0 % (0 of 1) |
Methyl | 41.2 % (47 of 114) | 42.1 % (24 of 57) | 40.4 % (23 of 57) |
1. FKBP12
MGVTVERIAP GDGKNFPKKG DKVTIHYVGT LENGDKFDSS RDRGSPFQCT IGVGQVIKGW DEGVTQLSVG EKARLICTHD YAYGERGYPG LIPPKATLNF EVELIKINSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FKBP12 | [U-98% 13C; U-98% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | sodium azide | natural abundance | 0.02 % |