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BMRB: 27738

8OXP

15N, 13C and 1H backbone resonance assignments of Human FKBP12 protein

Authors

Gobeil, S.M.C., Bobay, B.G., Spicer, L.D., Venters, R.A.

Assembly

FKBP12

Entity

1. FKBP12 (polymer, Thiol state: all free), 107 monomers, 11819.37 Da Detail

GVQVETISPG DGRTFPKRGQ TCVVHYTGML EDGKKFDSSR DRNKPFKFML GKQEVIRGWE EGVAQMSVGQ RAKLTISPDY AYGATGHPGI IPPHATLVFD VELLKLE

Formula weight

11819.37 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 53.1 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	53.1 % (659 of 1240)	36.7 % (238 of 648)	66.2 % (321 of 485)	93.5 % (100 of 107)
Backbone	97.3 % (611 of 628)	94.5 % (208 of 220)	98.4 % (303 of 308)	100.0 % (100 of 100)
Sidechain	19.8 % (140 of 706)	7.0 % (30 of 428)	40.6 % (110 of 271)	0.0 % (0 of 7)
Aromatic	4.1 % (4 of 98)	4.1 % (2 of 49)	4.2 % (2 of 48)	0.0 % (0 of 1)
Methyl	15.7 % (17 of 108)	7.4 % (4 of 54)	24.1 % (13 of 54)

1. FKBP12

GVQVETISPG DGRTFPKRGQ TCVVHYTGML EDGKKFDSSR DRNKPFKFML GKQEVIRGWE EGVAQMSVGQ RAKLTISPDY AYGATGHPGI IPPHATLVFD VELLKLE

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	FKBP12	[U-98% 13C; U-98% 15N]	0.7 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	sodium azide	natural abundance	0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.32 ppm, Outliers: 2 Detail

Release date

2018-12-19

Citation

¹⁵N, ¹³C and ¹H resonance assignments of FKBP12 proteins from the pathogenic fungi Mucor circinelloides and Aspergillus fumigatus
Gobeil, S.M.C., Bobay, B.G., Spicer, L.D., Venters, R.A.
Biomol. NMR Assign. (2019), 13, 207-212, PubMed 30707421 , DOI 10.1007/s12104-019-09878-x , Abstract

Related entities 1. FKBP12, : 1 : 69 : 5 : 24 : 264 entities Detail

Interaction partners 1. FKBP12, : 22 interactors Detail

More details for 22 interactors identified by 14 citations

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC