BMRB: 27836

Backbone 1H, 15N, and 13C Chemical Shifts of Myosin VI Medial Tail Domain

Authors

Barnes, C., Shen, Y., Ying, J., Takagi, Y., Torchia, D.A., Sellers, J.R., Bax, A.

Assembly

MT Domain

Entity

1. MT Domain (polymer, Thiol state: not present), 68 monomers, 8914.736 Da Detail

KQQEEEAERL RRIQEEMEKE RKRREEDEQR RRKEEEERRM KLEMEAKRKQ EEEERKKRED DEKRIQAE

Formula weight

8914.736 Da

Source organism

Sus scrofa

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 31.1 %, Completeness (bb): 64.2 % Detail

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Polymer type: polypeptide(D)

	Total	1H	13C	15N
All	31.1 % (275 of 883)	16.5 % (82 of 497)	40.4 % (126 of 312)	90.5 % (67 of 74)
Backbone	64.2 % (262 of 408)	52.2 % (71 of 136)	60.8 % (124 of 204)	98.5 % (67 of 68)
Sidechain	13.4 % (73 of 543)	3.0 % (11 of 361)	35.2 % (62 of 176)	0.0 % (0 of 6)
Methyl	13.6 % (3 of 22)	0.0 % (0 of 11)	27.3 % (3 of 11)

1. MT Domain

KQQEEEAERL RRIQEEMEKE RKRREEDEQR RRKEEEERRM KLEMEAKRKQ EEEERKKRED DEKRIQAE

Show sample condition

Sample #1

Solvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3, Details pH = 6.3, 20 C

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance		20 mM
2	EDTA	natural abundance		2 mM
3	MT Domain	[U-13C; U-15N; U-2H]		1 mM

Sample #2

Solvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 273 K, pH 6.3

#	Name	Isotope labeling	Type	Concentration
4	sodium phosphate	natural abundance		20 mM
5	EDTA	natural abundance		2 mM
6	MT Domain	[U-100% 2H; U-100% 15N]		1 mM

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Release date

2019-03-13

Citation

Remarkable Rigidity of the Single α-Helical Domain of Myosin-VI As Revealed by NMR Spectroscopy
Barnes, C., Shen, Y., Ying, J., Takagi, Y., Torchia, D.A., Sellers, J.R., Bax, A.
J. Am. Chem. Soc. (2019), 141, 9004-9017, PubMed 31117653 , DOI 10.1021/jacs.9b03116 , Abstract

Related entities 1. MT Domain, : 1 : 2 : 2 : 1 : 31 entities Detail

Interaction partners 1. MT Domain, : 2 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail