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NMR resonance assignments of the hazelnut allergen isoform Cor a 1.0403
Authors
Fuehrer, S., Zeindl, R., Tollinger, M.
Assembly
Cor a 1.0403
Entity
1. Cor a 1.0403 (polymer, Thiol state: all free), 160 monomers, 17395.60 Da Detail

GVFCYEDEAT SVIPPARLFK SFVLDADNLI PKVAPQHFTG AENLEGNGGP GTIKKITFAE GSEFKYMKHK VEEIDHANFK YCYSIIEGGP LGHTLEKISY EIKMAAAPHG GGSILKITSK YHTKGNASIS EEEIKAGKEK AAGLFKAVEA YLLAHPDTYC


Formula weight
17395.6 Da
Source organism
Corylus avellana
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.5 %, Completeness: 91.9 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.9 % (1690 of 1838)91.3 % (868 of 951)92.5 % (674 of 729)93.7 % (148 of 158)
Backbone94.7 % (894 of 944)93.3 % (306 of 328)96.1 % (446 of 464)93.4 % (142 of 152)
Sidechain90.0 % (934 of 1038)90.2 % (562 of 623)89.5 % (366 of 409)100.0 % (6 of 6)
Aromatic75.6 % (130 of 172)75.6 % (65 of 86)75.6 % (65 of 86)
Methyl97.0 % (161 of 166)98.8 % (82 of 83)95.2 % (79 of 83)

1. Cor a 1.0403

GVFCYEDEAT SVIPPARLFK SFVLDADNLI PKVAPQHFTG AENLEGNGGP GTIKKITFAE GSEFKYMKHK VEEIDHANFK YCYSIIEGGP LGHTLEKISY EIKMAAAPHG GGSILKITSK YHTKGNASIS EEEIKAGKEK AAGLFKAVEA YLLAHPDTYC

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9


#NameIsotope labelingTypeConcentration
1Cor a 1.0403[U-99% 13C; U-99% 15N]0.5 mM
2sodium phosphatenatural abundance20 mM
3DTTnatural abundance2 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9


#NameIsotope labelingTypeConcentration
4Cor a 1.0403[U-99% 15N]0.5 mM
5sodium phosphatenatural abundance20 mM
6DTTnatural abundance2 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 6Y3K, Strand ID: A Detail


Release date
2019-07-04
Citation
NMR resonance assignments of the four isoforms of the hazelnut allergen Cor a 1.04
Fuehrer, S., Zeindl, R., Tollinger, M.
Biomol. NMR Assign. (2019), 14, 45-49, PubMed 31691092 , DOI 10.1007/s12104-019-09918-6 ,
Related entities 1. Cor a 1.0403, : 1 : 1 : 200 entities Detail
Experiments performed 14 experiments Detail
Chemical shift validation 3 contents Detail