BMRBj - BMREntryMaster

Found 1 Document (2.329 seconds)

BMRB: 28028

3SY2

1H, 13C, 15N backbone and side-chain assignment of the native form of UbcH7 (UBE2L3)

Authors

Marousis, K.D., Seliami, A., Birkou, M., Spyroulias, G.A.

Assembly

E2-Conjugating Enzyme

Entity

1. E2-Conjugating Enzyme (polymer, Thiol state: all free), 154 monomers, 17861.35 Da Detail

MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD

Formula weight

17861.35 Da

Source organism

Exptl. method

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.9 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.9 % (1803 of 1900)	95.1 % (961 of 1010)	93.6 % (686 of 733)	99.4 % (156 of 157)
Backbone	98.4 % (882 of 896)	98.0 % (293 of 299)	98.5 % (450 of 457)	99.3 % (139 of 140)
Sidechain	92.8 % (1070 of 1153)	94.0 % (668 of 711)	90.6 % (385 of 425)	100.0 % (17 of 17)
Aromatic	55.6 % (79 of 142)	63.4 % (45 of 71)	46.4 % (32 of 69)	100.0 % (2 of 2)
Methyl	99.3 % (145 of 146)	98.6 % (72 of 73)	100.0 % (73 of 73)

1. UbcH7

MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK RPVD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	UbcH7	[U-99% 15N]		1.0 mM
2	K2HPO4/KH2PO4	natural abundance		50.0 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

Protein Blocks Logo

Calculated from 21 models in PDB: 6XXU, Strand ID: A Detail

Release date

2019-10-08

Citation

¹H, ¹³C, ¹⁵N backbone and side-chain resonance assignment of the native form of UbcH7 (UBE2L3) through solution NMR spectroscopy
Marousis, K.D., Birkou, M., Asimakopoulou, A., Spyroulias, G.A.
Biomol. NMR Assign. (2019), 14, 73-78, PubMed 31792831 , DOI 10.1007/s12104-019-09923-9 , Abstract

Related entities 1. E2-Conjugating Enzyme, : 1 : 8 : 1 : 14 : 257 entities Detail

Interaction partners 1. E2-Conjugating Enzyme, : 45 interactors Detail

More details for 45 interactors identified by 21 citations

Experiments performed 14 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	UbcH7	[U-99% 15N]		1.0 mM
2	K2HPO4/KH2PO4	natural abundance		50.0 mM

2 2D 1H-15N HSQC

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	UbcH7	[U-99% 15N]		1.0 mM
2	K2HPO4/KH2PO4	natural abundance		50.0 mM

3 3D HNHA

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	UbcH7	[U-99% 15N]		1.0 mM
2	K2HPO4/KH2PO4	natural abundance		50.0 mM

4 2D 1H-13C HSQC

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

5 3D HNCO

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

6 3D HNCA

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

7 3D HNCACB

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

8 3D HN(CO)CA

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

9 3D HN(CA)CO

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

10 3D CBCA(CO)NH

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

11 3D HBHA(CO)NH

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

12 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

13 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

14 3D HCCH-TOCSY

Instrument

Bruker Avance III HD - 700 MHz equipped with TCI cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	UbcH7	[U-98% 13C; U-98% 15N]		1.0 mM
4	K2HPO4/KH2PO4	natural abundance		50.0 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr28028_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr28028_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1957		100.0 (chain: 1, length: 154)
1	Chemical shift references	chemical_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 154, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 1116
  - ¹³C chemical shifts: 684
  - ¹⁵N chemical shifts: 157
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords E2 enzyme, Secondary Structure, Solution NMR Spectroscopy, UBC, UBE2L3, UbcH7