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BMRB: 28058

6SJW

NMR assignment of self-processing module of FrpC protein of Neisseria meningitidis loaded with calcium ions

Authors

Kuban, V., Zidek, L., Macek, P., Hritz, J., Bumba, L., M, F., K, N., K, N.

Assembly

4Ca.SPM

Entity

1. SPM (polymer, Thiol state: all free), 179 monomers, 18997.55 Da Detail

PLALDLDGDG IETVATKGFS GSLFDHNRDG IRTATGWVSA DDGLLVRDLN GNGIIDNGAE LFGDNTKLAD GSFAKHGYAA LAELDSNGDN IINAADAAFQ SLRVWQDLNQ DGISQANELR TLEELGIQSL DLAYKDVNKN LGNGNTLAQQ GSYTKTNGTT AKMGDLLLAA DNLHSRFLE

2. 4Ca.SPM, entity CA (non-polymer), 40.078 Da

Total weight

19037.629 Da

Max. entity weight

18997.55 Da

Source organism

Neisseria meningitidis

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 88.3 %, Completeness: 82.5 %, Completeness (bb): 85.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.5 % (1600 of 1939)	81.6 % (809 of 991)	83.4 % (621 of 745)	83.7 % (170 of 203)
Backbone	85.3 % (914 of 1072)	84.4 % (319 of 378)	85.5 % (441 of 516)	86.5 % (154 of 178)
Sidechain	80.4 % (824 of 1025)	79.9 % (490 of 613)	82.2 % (318 of 387)	64.0 % (16 of 25)
Aromatic	46.7 % (56 of 120)	46.7 % (28 of 60)	46.6 % (27 of 58)	50.0 % (1 of 2)
Methyl	93.0 % (199 of 214)	92.5 % (99 of 107)	93.5 % (100 of 107)

1. SPM

PLALDLDGDG IETVATKGFS GSLFDHNRDG IRTATGWVSA DDGLLVRDLN GNGIIDNGAE LFGDNTKLAD GSFAKHGYAA LAELDSNGDN IINAADAAFQ SLRVWQDLNQ DGISQANELR TLEELGIQSL DLAYKDVNKN LGNGNTLAQQ GSYTKTNGTT AKMGDLLLAA DNLHSRFLE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	SPM	[U-100% 13C; U-100% 15N]	0.4 mM
2	TRIS	natural abundance	5 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	0.1 %
5	Calcium chloride	natural abundance	10 mM

Heteronucl. T₁

124 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 850 MHz, Pressure 1 atm, Temperature 303 K, pH 7.4 Detail

Heteronucl. T₂

124 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 850 MHz, Pressure 1 atm, Temperature 303 K, pH 7.4 Detail

Heteronucl. NOE

124 NOE values in 1 lists
Value type peak height, Field strength (¹H) 850 MHz, Pressure 1 atm, Temperature 303 K, pH 7.4 Detail

Heteronucl. T₁/T₂

124 T₁/T₂ values in 1 lists
Field strength (¹H) 850 MHz, Pressure 1 atm, Temperature 303 K, pH 7.4 Detail

Release date

2020-01-02

Citation

Structural Basis of Ca²⁺-Dependent Self-Processing Activity of Repeat-in-Toxin Proteins
Macek, P., Kuban, V., Hritz, J., Nechvatalova, K., Nedbalcova, K., Faldyna, M., Zidek, L., Bumba, L.
mBio (2020), 11, e00226-20-e00226-20, PubMed 32184239 , DOI 10.1128/mBio.00226-20 , Abstract

Related entities 1. SPM, : 1 : 1 : 6 entities Detail

Experiments performed 12 experiments Detail

1 3D HNCO