Backbone and side-chain chemical shift assignments of a cellular FLICE-inhibitory protein (c-FLIPs)
MGRSDSAEVI HQVEEALDTD EKEMLLFLCR DVAIDVVPPN VRDLLDILRE RGKLSVGDLA ELLYRVRRFD LLKRILKMDR KAVETHLLRN PHLVSDYRVL MAEIGEDLDK SDVSSLIGLM KDYMGRGKIS KEKSFLDLVV ELEKLNLVAP DQLDLLEKCL KNIHRIDLKT KIQKYKQLEH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.3 % (1876 of 2253) | 82.6 % (970 of 1174) | 82.6 % (724 of 876) | 89.7 % (182 of 203) |
Backbone | 94.3 % (1039 of 1102) | 93.8 % (350 of 373) | 93.4 % (512 of 548) | 97.8 % (177 of 181) |
Sidechain | 75.7 % (1006 of 1329) | 77.4 % (620 of 801) | 75.3 % (381 of 506) | 22.7 % (5 of 22) |
Aromatic | 0.0 % (0 of 102) | 0.0 % (0 of 51) | 0.0 % (0 of 51) | |
Methyl | 96.0 % (238 of 248) | 97.6 % (121 of 124) | 94.4 % (117 of 124) |
1. c-FLPs
MGRSDSAEVI HQVEEALDTD EKEMLLFLCR DVAIDVVPPN VRDLLDILRE RGKLSVGDLA ELLYRVRRFD LLKRILKMDR KAVETHLLRN PHLVSDYRVL MAEIGEDLDK SDVSSLIGLM KDYMGRGKIS KEKSFLDLVV ELEKLNLVAP DQLDLLEKCL KNIHRIDLKT KIQKYKQLEH HHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 600 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Bruker Avance - 800 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | c-FLPs | [U-99% 13C; U-99% 15N] | 0.5 mM | |
2 | sodium phosphate | natural abundance | 60 mM | |
3 | NaCl | natural abundance | 50 mM |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr28061_3.str
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MGRSDSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVL ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ...SDSAEVIHQVEEALDTDEKEMLLFLCRDVAIDVVPPNVRDLLDILRERGKLSVGDLAELLYRVRRFDLLKRILKMDRKAVETHLLRNPHLVSDYRVL --------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 -------110-------120-------130-------140-------150-------160-------170-------180----- MAEIGEDLDKSDVSSLIGLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQLEHHHHHH ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| MAEIGEDLDKSDVSSLIGLMKDYMGRGKISKEKSFLDLVVELEKLNLVAPDQLDLLEKCLKNIHRIDLKTKIQKYKQLEHH -------110-------120-------130-------140-------150-------160-------170-------180-
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1174 | 956 | 81.4 |
13C chemical shifts | 876 | 717 | 81.8 |
15N chemical shifts | 203 | 179 | 88.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 373 | 351 | 94.1 |
13C chemical shifts | 370 | 342 | 92.4 |
15N chemical shifts | 181 | 174 | 96.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 801 | 605 | 75.5 |
13C chemical shifts | 506 | 375 | 74.1 |
15N chemical shifts | 22 | 5 | 22.7 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 130 | 126 | 96.9 |
13C chemical shifts | 130 | 121 | 93.1 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 51 | 0 | 0.0 |
13C chemical shifts | 51 | 0 | 0.0 |