NMR structure of a new G-quadruplex forming sequence within the KRAS proto-oncogene promoter region
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 92.6 % (188 of 203) | 92.6 % (188 of 203) |
Suger, PO4 | 96.1 % (148 of 154) | 96.1 % (148 of 154) |
Nucleobase | 81.6 % (40 of 49) | 81.6 % (40 of 49) |
Aromatic | 84.1 % (37 of 44) | 84.1 % (37 of 44) |
Methyl | 100.0 % (3 of 3) | 100.0 % (3 of 3) |
1. DNA (5'-D(*AP*GP*GP*GP*CP*GP*GP*TP*GP*TP*GP*GP*GP*AP*AP*TP*AP*GP*GP*GP*AP*A)-3')
AGGGCGGTGT GGGAATAGGG AASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 (±0.2) K, pH 6.6 (±0.1), Details 2 mM KRAS 22RT, 2 mM dG (5% 15N) KRAS 22RT 15N, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | KRAS 22RT | natural abundance | 2 mM | |
2 | KRAS 22RT 15N | dG (5% 15N) | 2 mM | |
3 | H2O | natural abundance | 90 % | |
4 | D2O | natural abundance | 10 % |