Solution Structure of DNA Dodecamer with 8-oxoguanine at 4th Position
Polymer type: polydeoxyribonucleotide
Total | 1H | 31P | |
---|---|---|---|
All | 60.8 % (73 of 120) | 67.0 % (73 of 109) | 0.0 % (0 of 11) |
Suger, PO4 | 61.4 % (54 of 88) | 70.1 % (54 of 77) | 0.0 % (0 of 11) |
Nucleobase | 59.4 % (19 of 32) | 59.4 % (19 of 32) | |
Aromatic | 77.3 % (17 of 22) | 77.3 % (17 of 22) | |
Methyl | 100.0 % (2 of 2) | 100.0 % (2 of 2) |
1. DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3')
CGCXAATTCG CGSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 50 mM sodium chloride, 10 mM potassium phosphate, 1 mM EDTA, 0.2 mM DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3'), 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3') | natural abundance | 0.2 mM | |
2 | EDTA | natural abundance | 1 mM | |
3 | potassium phosphate | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | D2O | natural abundance | 100 % |
Polymer type: polydeoxyribonucleotide
Total | 1H | 31P | |
---|---|---|---|
All | 34.6 % (83 of 240) | 38.1 % (83 of 218) | 0.0 % (0 of 22) |
Suger, PO4 | 30.7 % (54 of 176) | 35.1 % (54 of 154) | 0.0 % (0 of 22) |
Nucleobase | 45.3 % (29 of 64) | 45.3 % (29 of 64) | |
Aromatic | 47.7 % (21 of 44) | 47.7 % (21 of 44) | |
Methyl | 50.0 % (2 of 4) | 50.0 % (2 of 4) |
1. DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3')
CGCXAATTCG CGSolvent system 100% D2O, Pressure 1 atm, Temperature 278 K, pH 6.8, Details 50 mM sodium chloride, 10 mM potassium phosphate, 1 mM EDTA, 0.2 mM DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3'), 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3') | natural abundance | 0.2 mM | |
2 | EDTA | natural abundance | 1 mM | |
3 | potassium phosphate | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | D2O | natural abundance | 100 % |
Polymer type: polydeoxyribonucleotide
Total | 1H | 31P | |
---|---|---|---|
All | 24.7 % (89 of 360) | 25.4 % (83 of 327) | 18.2 % (6 of 33) |
Suger, PO4 | 22.7 % (60 of 264) | 23.4 % (54 of 231) | 18.2 % (6 of 33) |
Nucleobase | 30.2 % (29 of 96) | 30.2 % (29 of 96) | |
Aromatic | 31.8 % (21 of 66) | 31.8 % (21 of 66) | |
Methyl | 33.3 % (2 of 6) | 33.3 % (2 of 6) |
1. DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3')
CGCXAATTCG CGSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 50 mM sodium chloride, 10 mM potassium phosphate, 1 mM EDTA, 0.2 mM DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3'), 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*CP*GP*CP*(8OG)P*AP*AP*TP*TP*CP*GP*CP*G)-3') | natural abundance | 0.2 mM | |
2 | EDTA | natural abundance | 1 mM | |
3 | potassium phosphate | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 50 mM | |
5 | D2O | natural abundance | 100 % |