Solution structure of Ras Binding Domain (RBD) of B-Raf
GSLEVLFQGP SPQKPIVRVF LPNKQRTVVP ARCGVTVRDS LKKALMMRGL IPECCAVYRI QDGEKKPIGW DTDISWLTGE ELHVEVLENV PL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.1 % (950 of 1091) | 86.2 % (493 of 572) | 87.8 % (375 of 427) | 89.1 % (82 of 92) |
Backbone | 89.7 % (481 of 536) | 89.6 % (164 of 183) | 89.2 % (240 of 269) | 91.7 % (77 of 84) |
Sidechain | 81.6 % (522 of 640) | 80.2 % (312 of 389) | 84.4 % (205 of 243) | 62.5 % (5 of 8) |
Aromatic | 67.9 % (38 of 56) | 67.9 % (19 of 28) | 65.4 % (17 of 26) | 100.0 % (2 of 2) |
Methyl | 83.1 % (98 of 118) | 83.1 % (49 of 59) | 83.1 % (49 of 59) |
1. entity 1
GSLEVLFQGP SPQKPIVRVF LPNKQRTVVP ARCGVTVRDS LKKALMMRGL IPECCAVYRI QDGEKKPIGW DTDISWLTGE ELHVEVLENV PLSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 200 uM [U-99% 13C; U-99% 15N] B-RAF RBD:Rigosertib Complex II, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | B-RAF RBD:Rigosertib Complex II | [U-99% 13C; U-99% 15N] | 200 uM | |
2 | 6FS ligand | [U-99% 13C; U-99% 15N] | 200 uM | |
3 | NaCl | natural abundance | 200 mM |