Solution structure of the de novo miniprotein EHE_06
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.3 % (305 of 405) | 85.8 % (188 of 219) | 54.7 % (82 of 150) | 97.2 % (35 of 36) |
Backbone | 74.3 % (156 of 210) | 90.3 % (65 of 72) | 55.3 % (57 of 103) | 97.1 % (34 of 35) |
Sidechain | 76.3 % (174 of 228) | 83.7 % (123 of 147) | 62.5 % (50 of 80) | 100.0 % (1 of 1) |
Aromatic | 50.0 % (8 of 16) | 100.0 % (8 of 8) | 0.0 % (0 of 8) | |
Methyl | 100.0 % (18 of 18) | 100.0 % (9 of 9) | 100.0 % (9 of 9) |
1. W35
CKQRRRYRGS EEECRKYAEE LSRRTGCEVE VECETSolvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 293 (±0.5) K, pH 4.8 (±0.2), Details 50 mM sodium chloride, 25 mM sodium acetate, 1 mM [U-99% 15N] W35, 93% H2O/7% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | W35 | [U-99% 15N] | 1 (±0.2) mM | |
2 | sodium acetate | natural abundance | 25 (±1.0) mM | |
3 | sodium chloride | natural abundance | 50 (±2.0) mM | |
4 | H2O | natural abundance | 93 % | |
5 | D2O | natural abundance | 7 % |