The NMR Solution Structure of RPA3313
MGSSHHHHHH SSGRENLYFQ GMGKAYYDIV GSDNRWGIRH DDDPTGDYSS KEAAFEAACA AASNAIKFGH EVRITVPGND GSETNLGVIT N
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 67.3 % (655 of 973) | 67.5 % (336 of 498) | 66.1 % (250 of 378) | 71.1 % (69 of 97) |
Backbone | 75.3 % (408 of 542) | 73.3 % (140 of 191) | 76.0 % (199 of 262) | 77.5 % (69 of 89) |
Sidechain | 60.3 % (308 of 511) | 63.8 % (196 of 307) | 57.1 % (112 of 196) | 0.0 % (0 of 8) |
Aromatic | 22.6 % (24 of 106) | 45.3 % (24 of 53) | 0.0 % (0 of 52) | 0.0 % (0 of 1) |
Methyl | 87.1 % (61 of 70) | 80.0 % (28 of 35) | 94.3 % (33 of 35) |
1. Uncharacterized protein
MGSSHHHHHH SSGRENLYFQ GMGKAYYDIV GSDNRWGIRH DDDPTGDYSS KEAAFEAACA AASNAIKFGH EVRITVPGND GSETNLGVIT NSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.6, Details 18 mM NA MES, 80 mM sodium chloride, 0.1 % sodium azide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RPA3313 | none | 1 mM | |
2 | MES | natural abundance | 18 mM | |
3 | sodium azide | natural abundance | 0.1 % | |
4 | sodium chloride | natural abundance | 80 mM |