Solution structure of the TRIM21 B-box2 (B2)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.6 % (458 of 511) | 89.6 % (240 of 268) | 90.8 % (178 of 196) | 85.1 % (40 of 47) |
Backbone | 90.7 % (243 of 268) | 90.3 % (84 of 93) | 91.6 % (120 of 131) | 88.6 % (39 of 44) |
Sidechain | 89.1 % (253 of 284) | 89.1 % (156 of 175) | 90.6 % (96 of 106) | 33.3 % (1 of 3) |
Aromatic | 73.7 % (28 of 38) | 78.9 % (15 of 19) | 66.7 % (12 of 18) | 100.0 % (1 of 1) |
Methyl | 100.0 % (38 of 38) | 100.0 % (19 of 19) | 100.0 % (19 of 19) |
1. E3 ubiquitin-protein ligase TRIM21
GTQGERCAVH GERLHLFCEK DGKALCWVCA QSRKHRDHAM VPLEESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 450 uM [U-13C; U-15N] TRIM21 Bbox2, 50 mM TRIS, 150 mM sodium chloride, 10 mM beta-mercaptoethanol, 10 uM ZINC ION, 10 % glycerol, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | TRIM21 Bbox2 | [U-13C; U-15N] | 450 uM | |
2 | TRIS | natural abundance | 50 mM | |
3 | ZINC ION | natural abundance | 10 uM | |
4 | beta-mercaptoethanol | natural abundance | 10 mM | |
5 | glycerol | natural abundance | 10 % | |
6 | sodium chloride | natural abundance | 150 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 450 uM [U-13C; U-15N] TRIM21 Bbox2, 50 mM deuterated TRIS, 150 mM sodium chloride, 10 mM beta-mercaptoethanol, 10 uM ZINC ION, 10 % glycerol, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | TRIM21 Bbox2 | [U-13C; U-15N] | 450 uM | |
8 | TRIS | deuterated | 50 mM | |
9 | ZINC ION | natural abundance | 10 uM | |
10 | beta-mercaptoethanol | natural abundance | 10 mM | |
11 | glycerol | natural abundance | 10 % | |
12 | sodium chloride | natural abundance | 150 mM |