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NMR structure of the HLTF HIRAN domain
Authors
Bezsonova, I., Neculai, D., Korzhnev, D., Weigelt, J., Bountra, C., Edwards, A., Arrowsmith, C., Dhe-Paganon, S., Structural Genomics Consortium (SGC), S.
Assembly
Helicase-like transcription factor (E.C.3.6.4.-,6.3.2.-)
Entity
1. Helicase-like transcription factor (E.C.3.6.4.-,6.3.2.-) (polymer, Thiol state: not present), 122 monomers, 13514.17 Da Detail

GSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KL


Formula weight
13514.17 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts, spectral_peak_list
Chem. Shift Complete
Sequence coverage: 99.2 %, Completeness: 90.1 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All90.1 % (1279 of 1420)90.0 % (665 of 739)89.2 % (485 of 544)94.2 % (129 of 137)
Backbone95.4 % (691 of 724)96.4 % (243 of 252)94.1 % (333 of 354)97.5 % (115 of 118)
Sidechain85.7 % (691 of 806)86.7 % (422 of 487)85.0 % (255 of 300)73.7 % (14 of 19)
Aromatic52.7 % (58 of 110)60.0 % (33 of 55)44.4 % (24 of 54)100.0 % (1 of 1)
Methyl97.0 % (128 of 132)95.5 % (63 of 66)98.5 % (65 of 66)

1. entity 1

GSDEEVDSVL FGSLRGHVVG LRYYTGVVNN NEMVALQRDP NNPYDKNAIK VNNVNGNQVG HLKKELAGAL AYIMDNKLAQ IEGVVPFGAN NAFTMPLHMT FWGKEENRKA VSDQLKKHGF KL

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 1 mM [U-99% 13C; U-99% 15N] HLTF HIRAN domain, 20 mM sodium phosphate, 100 mM sodium chloride, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1HLTF HIRAN domain[U-99% 13C; U-99% 15N]1 mM
2sodium chloridenatural abundance100 mM
3sodium phosphatenatural abundance20 mM

LACS Plot; CA
Referencing offset: -1.08 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -1.08 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: 0.31 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: -0.48 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 5K5F, Strand ID: A Detail


Release date
2016-06-05
Citation
Solution NMR structure of the HLTF HIRAN domain: a conserved module in SWI2/SNF2 DNA damage tolerance proteins
Korzhnev, D.M., Neculai, D., Dhe-Paganon, S., Arrowsmith, C.H., Bezsonova, I.
J. Biomol. NMR (2016), 66, 209-219, PubMed 27771863 , DOI 10.1007/s10858-016-0070-9 ,
Related entities 1. Helicase-like transcription factor (E.C.3.6.4.-,6.3.2.-), : 1 : 1 : 6 : 11 entities Detail
Interaction partners 1. Helicase-like transcription factor (E.C.3.6.4.-,6.3.2.-), : 30 interactors Detail
Experiments performed 12 experiments Detail
NMR combined restraints 5 contents Detail
Keywords DNA replication, DNA-binding, PSI-Biology, Protein Structure Initiative, SGC, Structural Genomics, Structural Genomics Consortium