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BMRB: 30100

5K6P

The NMR structure of the m domain tri-helix bundle and C2 of human cardiac Myosin Binding Protein C

Authors

Michie, K.A., Kwan, A.H., Tung, C.S., Guss, J.M., Trewhella, J.

Assembly

Myosin-binding protein C, cardiac-type

Entity

1. Myosin-binding protein C, cardiac-type (polymer), 137 monomers, 15473.50 Da Detail

GPGSEDVWEI LRQAPPSEYE RIAFQYGVTD LRGMLKRLKG MRRDEKKSTA FQKKLEPAYQ VSKGHKIRLT VELADHDAEV KWLKNGQEIQ MSGSKYIFES IGAKRTLTIS QCSLADDAAY QCVVGGEKCS TELFVKE

Formula weight

15473.5 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 95.5 %, Completeness (bb): 97.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.5 % (1545 of 1617)	96.4 % (819 of 850)	93.6 % (583 of 623)	99.3 % (143 of 144)
Backbone	97.8 % (796 of 814)	96.8 % (272 of 281)	98.0 % (392 of 400)	99.2 % (132 of 133)
Sidechain	94.2 % (875 of 929)	96.1 % (547 of 569)	90.8 % (317 of 349)	100.0 % (11 of 11)
Aromatic	64.3 % (72 of 112)	80.4 % (45 of 56)	46.3 % (25 of 54)	100.0 % (2 of 2)
Methyl	97.8 % (133 of 136)	98.5 % (67 of 68)	97.1 % (66 of 68)

1. Myosin-binding protein C, cardiac-type

GPGSEDVWEI LRQAPPSEYE RIAFQYGVTD LRGMLKRLKG MRRDEKKSTA FQKKLEPAYQ VSKGHKIRLT VELADHDAEV KWLKNGQEIQ MSGSKYIFES IGAKRTLTIS QCSLADDAAY QCVVGGEKCS TELFVKE

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 412 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	CaCl2	natural abundance	1 mM
2	DSS	natural abundance	10 mM
3	MES	natural abundance	10 mM
4	Na azide	natural abundance	1 mM
5	NaCl	natural abundance	50 mM
6	TCEP	natural abundance	2 mM
7	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	412 uM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 920 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
10	CaCl2	natural abundance	1 mM
11	DSS	natural abundance	10 mM
12	MES	natural abundance	10 mM
13	Na azide	natural abundance	1 mM
14	NaCl	natural abundance	50 mM
15	TCEP	natural abundance	2 mM
16	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	920 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 550 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 1 mM Na azide, 2 mM TCEP, 10 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
19	CaCl2	natural abundance	1 mM
20	DSS	natural abundance	10 mM
21	MES	natural abundance	10 mM
22	Na azide	natural abundance	1 mM
23	NaCl	natural abundance	50 mM
24	TCEP	natural abundance	2 mM
25	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	550 uM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %

Sample #4

Solvent system 99% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 500 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O

#	Name	Isotope labeling	Concentration
28	CaCl2	natural abundance	1 mM
29	DSS	natural abundance	10 mM
30	MES	natural abundance	10 mM
31	Na azide	natural abundance	1 mM
32	NaCl	natural abundance	50 mM
33	TCEP	natural abundance	2 mM
34	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	500 uM
35	H2O	natural abundance	1 %
36	D2O	natural abundance	99 %

Sample #5

Solvent system 99% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 800 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O

#	Name	Isotope labeling	Concentration
37	CaCl2	natural abundance	1 mM
38	DSS	natural abundance	10 mM
39	MES	natural abundance	10 mM
40	Na azide	natural abundance	1 mM
41	NaCl	natural abundance	50 mM
42	TCEP	natural abundance	2 mM
43	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	800 uM
44	H2O	natural abundance	1 %
45	D2O	natural abundance	99 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -2.71 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -2.71 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 6 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -2.63 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5K6P, Strand ID: A Detail

Release date

2016-11-03

Citation

A Highly Conserved Yet Flexible Linker Is Part of a Polymorphic Protein-Binding Domain in Myosin-Binding Protein C
Michie, K.A., Kwan, A.H., Tung, C.S., Guss, J.M., Trewhella, J.
Structure (2016), 24, 2000-2007, PubMed 27720588 , DOI 10.1016/j.str.2016.08.018 , Abstract

Related entities 1. Myosin-binding protein C, cardiac-type, : 1 : 3 : 50 entities Detail

Interaction partners 1. Myosin-binding protein C, cardiac-type, : 5 interactors Detail

More details for 5 interactors identified by 4 citations

Experiments performed 18 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 920 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
10	CaCl2	natural abundance	1 mM
11	DSS	natural abundance	10 mM
12	MES	natural abundance	10 mM
13	Na azide	natural abundance	1 mM
14	NaCl	natural abundance	50 mM
15	TCEP	natural abundance	2 mM
16	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	920 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

2 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 412 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
1	CaCl2	natural abundance	1 mM
2	DSS	natural abundance	10 mM
3	MES	natural abundance	10 mM
4	Na azide	natural abundance	1 mM
5	NaCl	natural abundance	50 mM
6	TCEP	natural abundance	2 mM
7	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	412 uM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

3 HCC(CO)NH TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
10	CaCl2	natural abundance	1 mM
11	DSS	natural abundance	10 mM
12	MES	natural abundance	10 mM
13	Na azide	natural abundance	1 mM
14	NaCl	natural abundance	50 mM
15	TCEP	natural abundance	2 mM
16	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	920 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

4 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	CaCl2	natural abundance	1 mM
2	DSS	natural abundance	10 mM
3	MES	natural abundance	10 mM
4	Na azide	natural abundance	1 mM
5	NaCl	natural abundance	50 mM
6	TCEP	natural abundance	2 mM
7	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	412 uM
8	H2O	natural abundance	95 %
9	D2O	natural abundance	5 %

5 2D HBCBCGCDCEHE

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 99% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 500 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O

#	Name	Isotope labeling	Concentration
28	CaCl2	natural abundance	1 mM
29	DSS	natural abundance	10 mM
30	MES	natural abundance	10 mM
31	Na azide	natural abundance	1 mM
32	NaCl	natural abundance	50 mM
33	TCEP	natural abundance	2 mM
34	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	500 uM
35	H2O	natural abundance	1 %
36	D2O	natural abundance	99 %

6 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 550 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 1 mM Na azide, 2 mM TCEP, 10 mM DSS, 95% H2O/5% D2O

#	Name	Isotope labeling	Concentration
19	CaCl2	natural abundance	1 mM
20	DSS	natural abundance	10 mM
21	MES	natural abundance	10 mM
22	Na azide	natural abundance	1 mM
23	NaCl	natural abundance	50 mM
24	TCEP	natural abundance	2 mM
25	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	550 uM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %

7 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
10	CaCl2	natural abundance	1 mM
11	DSS	natural abundance	10 mM
12	MES	natural abundance	10 mM
13	Na azide	natural abundance	1 mM
14	NaCl	natural abundance	50 mM
15	TCEP	natural abundance	2 mM
16	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	920 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

8 CC(CO)NH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
10	CaCl2	natural abundance	1 mM
11	DSS	natural abundance	10 mM
12	MES	natural abundance	10 mM
13	Na azide	natural abundance	1 mM
14	NaCl	natural abundance	50 mM
15	TCEP	natural abundance	2 mM
16	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	920 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

9 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
10	CaCl2	natural abundance	1 mM
11	DSS	natural abundance	10 mM
12	MES	natural abundance	10 mM
13	Na azide	natural abundance	1 mM
14	NaCl	natural abundance	50 mM
15	TCEP	natural abundance	2 mM
16	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	920 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

10 3D 13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
19	CaCl2	natural abundance	1 mM
20	DSS	natural abundance	10 mM
21	MES	natural abundance	10 mM
22	Na azide	natural abundance	1 mM
23	NaCl	natural abundance	50 mM
24	TCEP	natural abundance	2 mM
25	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	550 uM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %

11 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 99% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 800 uM [U-99% 13C; U-99% 15N] human cMyBP-C (319-451), 10 mM MES, 1 mM CaCl2, 50 mM NaCl, 2 mM TCEP, 1 mM Na azide, 10 mM DSS, 99% D2O

#	Name	Isotope labeling	Concentration
37	CaCl2	natural abundance	1 mM
38	DSS	natural abundance	10 mM
39	MES	natural abundance	10 mM
40	Na azide	natural abundance	1 mM
41	NaCl	natural abundance	50 mM
42	TCEP	natural abundance	2 mM
43	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	800 uM
44	H2O	natural abundance	1 %
45	D2O	natural abundance	99 %

12 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
19	CaCl2	natural abundance	1 mM
20	DSS	natural abundance	10 mM
21	MES	natural abundance	10 mM
22	Na azide	natural abundance	1 mM
23	NaCl	natural abundance	50 mM
24	TCEP	natural abundance	2 mM
25	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	550 uM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %

13 3D 13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
28	CaCl2	natural abundance	1 mM
29	DSS	natural abundance	10 mM
30	MES	natural abundance	10 mM
31	Na azide	natural abundance	1 mM
32	NaCl	natural abundance	50 mM
33	TCEP	natural abundance	2 mM
34	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	500 uM
35	H2O	natural abundance	1 %
36	D2O	natural abundance	99 %

14 3D HCC TOCSY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
37	CaCl2	natural abundance	1 mM
38	DSS	natural abundance	10 mM
39	MES	natural abundance	10 mM
40	Na azide	natural abundance	1 mM
41	NaCl	natural abundance	50 mM
42	TCEP	natural abundance	2 mM
43	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	800 uM
44	H2O	natural abundance	1 %
45	D2O	natural abundance	99 %

15 2D HBCBCGCDHD

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
28	CaCl2	natural abundance	1 mM
29	DSS	natural abundance	10 mM
30	MES	natural abundance	10 mM
31	Na azide	natural abundance	1 mM
32	NaCl	natural abundance	50 mM
33	TCEP	natural abundance	2 mM
34	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	500 uM
35	H2O	natural abundance	1 %
36	D2O	natural abundance	99 %

16 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
10	CaCl2	natural abundance	1 mM
11	DSS	natural abundance	10 mM
12	MES	natural abundance	10 mM
13	Na azide	natural abundance	1 mM
14	NaCl	natural abundance	50 mM
15	TCEP	natural abundance	2 mM
16	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	920 uM
17	H2O	natural abundance	95 %
18	D2O	natural abundance	5 %

17 3D hCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
37	CaCl2	natural abundance	1 mM
38	DSS	natural abundance	10 mM
39	MES	natural abundance	10 mM
40	Na azide	natural abundance	1 mM
41	NaCl	natural abundance	50 mM
42	TCEP	natural abundance	2 mM
43	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	800 uM
44	H2O	natural abundance	1 %
45	D2O	natural abundance	99 %

18 3D HcCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
19	CaCl2	natural abundance	1 mM
20	DSS	natural abundance	10 mM
21	MES	natural abundance	10 mM
22	Na azide	natural abundance	1 mM
23	NaCl	natural abundance	50 mM
24	TCEP	natural abundance	2 mM
25	human cMyBP-C (319-451)	[U-99% 13C; U-99% 15N]	550 uM
26	H2O	natural abundance	95 %
27	D2O	natural abundance	5 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_30100_5k6p.nef
Input source #2: Coordindates	5k6p.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---320-------330-------340-------350-------360-------370-------380-------390-------400-------410----
GPGSEDVWEILRQAPPSEYERIAFQYGVTDLRGMLKRLKGMRRDEKKSTAFQKKLEPAYQVSKGHKIRLTVELADHDAEVKWLKNGQEIQMSGSKYIFES
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GPGSEDVWEILRQAPPSEYERIAFQYGVTDLRGMLKRLKGMRRDEKKSTAFQKKLEPAYQVSKGHKIRLTVELADHDAEVKWLKNGQEIQMSGSKYIFES
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

---420-------430-------440-------450-
IGAKRTLTISQCSLADDAAYQCVVGGEKCSTELFVKE
|||||||||||||||||||||||||||||||||||||
IGAKRTLTISQCSLADDAAYQCVVGGEKCSTELFVKE
-------110-------120-------130-------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	137	0	0	100.0

Content subtype: combined_30100_5k6p.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1561		99.3 (chain: A, length: 137)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	5381 (1)	noe	98.5 (chain: A, length: 137)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	206 (206)	.	89.1 (chain: A, length: 137)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 137, Sequence coverage: 99.3%
- Total number of assignments
  - ¹H chemical shifts: 832
  - ¹³C chemical shifts: 582
  - ¹⁵N chemical shifts: 147
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
343	THR	HG1	0.803

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	850	831	97.8
¹³C chemical shifts	623	582	93.4
¹⁵N chemical shifts	152	147	96.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	281	278	98.9
¹³C chemical shifts	274	265	96.7
¹⁵N chemical shifts	133	132	99.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	569	553	97.2
¹³C chemical shifts	349	317	90.8
¹⁵N chemical shifts	19	15	78.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	71	70	98.6
¹³C chemical shifts	71	68	95.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	56	45	80.4
¹³C chemical shifts	54	25	46.3
¹⁵N chemical shifts	2	2	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 137, Sequence coverage: 98.5%
- Total number of restraints: 5381
- Weight of restraints: 1.0 (5381)
- Potential type of restraints: square-well-parabolic (5381)
- Range of distance target values: 1.47, 3.2 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 137, Sequence coverage: 89.1%
- Total number of restraints: 206
- Total number of restraints per polymer type: 206
- Weight of restraints: 1.0 (206)
- Potential type of restraints: square-well-parabolic (206)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

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Found 1 Document (1.057 seconds)

BMRB: 30100

Sample #1

Sample #2

Sample #3

Sample #4

Sample #5

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Myosin-binding protein C, cardiac-type, : 1 : 3 : 50 entities Detail

Interaction partners 1. Myosin-binding protein C, cardiac-type, : 5 interactors Detail

Experiments performed 18 experiments Detail

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Instrument

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Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

NMR combined restraints 4 contents Detail