Structural characterization of melanoregulin (mreg): the protein involved in regulation of cell pigmentation
EFSAGLRRWL RSACCCCPCR CLEEPARPEK EPLVSGNNPY SSFGATLERD DEKNLWSMPH DVSHTEADDD RILYNLIVIR NQQTKDSEEW QRLNYDIYTL RQIRREVRNR WRRILEDLGF QREADSLLSV TKLSTMSDSK NTRKAREMLL KLAEETSIFP ASWELSERYL LVVDRLIALD AAEDFFKIAS QMYPKKPGVP CLVDGQRKLH CLPFPSP
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 53.9 % (1399 of 2594) | 48.8 % (667 of 1367) | 57.3 % (575 of 1003) | 70.1 % (157 of 224) |
Backbone | 77.4 % (987 of 1276) | 78.2 % (334 of 427) | 76.9 % (496 of 645) | 77.0 % (157 of 204) |
Sidechain | 37.7 % (577 of 1529) | 35.4 % (333 of 940) | 42.9 % (244 of 569) | 0.0 % (0 of 20) |
Aromatic | 9.5 % (18 of 190) | 13.7 % (13 of 95) | 5.6 % (5 of 90) | 0.0 % (0 of 5) |
Methyl | 52.8 % (115 of 218) | 53.2 % (58 of 109) | 52.3 % (57 of 109) |
1. Melanoregulin
EFSAGLRRWL RSACCCCPCR CLEEPARPEK EPLVSGNNPY SSFGATLERD DEKNLWSMPH DVSHTEADDD RILYNLIVIR NQQTKDSEEW QRLNYDIYTL RQIRREVRNR WRRILEDLGF QREADSLLSV TKLSTMSDSK NTRKAREMLL KLAEETSIFP ASWELSERYL LVVDRLIALD AAEDFFKIAS QMYPKKPGVP CLVDGQRKLH CLPFPSPSolvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 15N] melanoregulin, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | melanoregulin | [U-99% 15N] | 0.150 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | natural abundance | 10 % | |
4 | L-Arginine | natural abundance | 5 mM | |
5 | L-Glutamine | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 13C; U-99% 15N] melanoregulin, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | melanoregulin | [U-99% 13C; U-99% 15N] | 0.150 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % | |
9 | L-Arginine | natural abundance | 5 mM | |
10 | L-Glutamine | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM U-15;U-13C;U-2H melanoregulin, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | melanoregulin | U-15;U-13C;U-2H | 0.150 mM | |
12 | H2O | natural abundance | 90 % | |
13 | D2O | natural abundance | 10 % | |
14 | L-Arginine | natural abundance | 5 mM | |
15 | L-Glutamine | natural abundance | 5 mM |
Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM 15N/13C/2H/ILV-CH3 melanoregulin, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
16 | melanoregulin | 15N/13C/2H/ILV-CH3 | 0.150 mM | |
17 | D2O | natural abundance | 100 % | |
18 | L-Arginine | natural abundance | 5 mM | |
19 | L-Glutamine | natural abundance | 5 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 303 (±0.2) K, pH 6.3 (±0.05), Details 0.150 mM [U-99% 15N] melanoregulin, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
20 | melanoregulin | [U-99% 15N] | 0.150 mM | |
21 | H2O | natural abundance | 90 % | |
22 | D2O | natural abundance | 10 % | |
23 | L-Arginine | natural abundance | 5 mM | |
24 | L-Glutamine | natural abundance | 5 mM |