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Connexin 26 G12R mutant NMR structure
Authors
Dowd, T.L., Bargiello, T.A.
Assembly
Gap junction beta-2 protein
Entity
1. Gap junction beta-2 protein (polymer, Thiol state: not present), 23 monomers, 2468.829 Da Detail

XMDWGTLQTI LGRVNKHSTS IGK


Formula weight
2468.829 Da
Source organism
Homo sapiens
Exptl. method
solution NMR
Refine. method
simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.7 %, Completeness: 66.1 %, Completeness (bb): 76.4 % Detail

Polymer type: polypeptide(L)

Total1H13C
All66.1 % (152 of 230)84.1 % (111 of 132)41.8 % (41 of 98)
Backbone76.4 % (84 of 110)91.5 % (43 of 47)65.1 % (41 of 63)
Sidechain62.6 % (87 of 139)80.0 % (68 of 85)35.2 % (19 of 54)
Aromatic53.3 % (8 of 15)100.0 % (8 of 8) 0.0 % (0 of 7)
Methyl50.0 % (13 of 26)100.0 % (13 of 13) 0.0 % (0 of 13)

1. entity 1

XMDWGTLQTI LGRVNKHSTS IGK

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 288 (±0.2) K, pH 7.0 (±0.05), Details 1.0 mM NA ACE-MET-ASP-TRP-GLY-THR-LEU-GLN-THR-ILE-LEU-GLY-ARG-VAL-ASN-LYS-HIS-SER-THR-SER-ILE-GLY-LYS, 100 mM KCL, 100 uM TSP, 90% H2O/10% D2O


#NameIsotope labelingTypeConcentration
1entity_1natural abundance1.0 (±0.1) mM
2KCLnatural abundance100 (±0.1) mM
3TSPnatural abundance100 (±2.0) uM

Protein Blocks Logo
Calculated from 20 models in PDB: 5KJG, Strand ID: A Detail


Release date
2016-09-22
Citation
Structural studies of N-terminal mutants of Connexin 26 and Connexin 32 using (1)H NMR spectroscopy
Batir, Y., Bargiello, T.A., Dowd, T.L.
Arch. Biochem. Biophys. (2016), 608, 8-19, PubMed 27378082 , DOI 10.1016/j.abb.2016.06.019 ,
Related entities 1. Gap junction beta-2 protein, : 1 : 11 : 102 entities Detail
Interaction partners 1. Gap junction beta-2 protein, : 2 interactors Detail
Experiments performed 3 experiments Detail
NMR combined restraints 3 contents Detail
Keywords Connexin 26 G12R Mutant, TRANSPORT PROTEIN