Notch1 transmembrane and associated juxtamembrane segment
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 33.9 % (243 of 716) | 16.2 % (60 of 371) | 47.9 % (137 of 286) | 78.0 % (46 of 59) |
Backbone | 67.2 % (231 of 344) | 41.0 % (48 of 117) | 79.2 % (137 of 173) | 85.2 % (46 of 54) |
Sidechain | 13.3 % (57 of 427) | 5.1 % (13 of 254) | 26.2 % (44 of 168) | 0.0 % (0 of 5) |
Aromatic | 0.0 % (0 of 102) | 0.0 % (0 of 51) | 0.0 % (0 of 50) | 0.0 % (0 of 1) |
Methyl | 8.3 % (5 of 60) | 0.0 % (0 of 30) | 16.7 % (5 of 30) |
1. entity 1
MGHHHHHHVQ SETVEPPPPA QLHFMYVAAA AFVLLFFVGC GVLLSRKRRR QHGQLWFPESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 318 K, pH 5.5, Details 350 uM [U-99% 15N] Notch1, 150 mg/mL DHPC/DMPC bicelle, 1 mM EDTA, 65 mM imidazole, 2 mM DTT, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DHPC/DMPC bicelle | natural abundance | 150 mg/mL | |
2 | DTT | natural abundance | 2 mM | |
3 | EDTA | natural abundance | 1 mM | |
4 | Notch1 | [U-99% 15N] | 350 uM | |
5 | imidazole | natural abundance | 65 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 318 K, pH 5.5, Details 500 uM [U-99% 13C; U-99% 15N] Notch1, 150 mg/mL DHPC/DMPC bicelle, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | DHPC/DMPC bicelle | natural abundance | 150 mg/mL | |
7 | Notch1 | [U-99% 13C; U-99% 15N] | 500 uM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 318 K, pH 5.5, Details 500 uM [U-99% 13C; U-99% 15N] Notch1, 150 mg/mL DHPC/DMPC, 1 mM EDTA, 65 mM imidazole, 2 mM DTT, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | DHPC/DMPC | natural abundance | 150 mg/mL | |
9 | DTT | natural abundance | 2 mM | |
10 | EDTA | natural abundance | 1 mM | |
11 | Notch1 | [U-99% 13C; U-99% 15N] | 500 uM | |
12 | imidazole | natural abundance | 65 mM |