Solution Structure of SinI, antagonist to the master biofilm-regulator SinR in Bacillus subtilis
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.8 % (642 of 748) | 86.0 % (339 of 394) | 87.5 % (252 of 288) | 77.3 % (51 of 66) |
Backbone | 89.2 % (330 of 370) | 87.1 % (108 of 124) | 91.4 % (171 of 187) | 86.4 % (51 of 59) |
Sidechain | 84.3 % (370 of 439) | 85.6 % (231 of 270) | 85.8 % (139 of 162) | 0.0 % (0 of 7) |
Aromatic | 46.4 % (26 of 56) | 46.4 % (13 of 28) | 48.1 % (13 of 27) | 0.0 % (0 of 1) |
Methyl | 89.3 % (50 of 56) | 89.3 % (25 of 28) | 89.3 % (25 of 28) |
1. Protein SinI
GSHMASMKNA KQEHFELDQE WVELMVEAKE ANISPEEIRK YLLLNKKSAH PGPAARSHTV NPFSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6, Details 1 mM [U-15N] SinI, 20 mM MES, 200 mM sodium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MES | natural abundance | 20 mM | |
2 | NaCl | natural abundance | 200 mM | |
3 | SinI | [U-15N] | 1 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6, Details 1 mM [U-13C; U-15N] SinI, 20 mM MES, 200 mM NaCl, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | MES | natural abundance | 20 mM | |
7 | NaCl | natural abundance | 200 mM | |
8 | SinI | [U-13C; U-15N] | 1 mM | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6, Details 1 mM [U-13C; U-15N] SinI, 20 mM MES, 200 mM NaCl, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | MES | natural abundance | 20 mM | |
12 | NaCl | natural abundance | 200 mM | |
13 | SinI | [U-13C; U-15N] | 1 mM | |
14 | D2O | natural abundance | 100 % |