Solution structure of Serine 65 phosphorylated UBL domain from parkin
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.0 % (816 of 917) | 85.4 % (409 of 479) | 93.8 % (332 of 354) | 89.3 % (75 of 84) |
Backbone | 97.1 % (431 of 444) | 98.0 % (147 of 150) | 96.4 % (214 of 222) | 97.2 % (70 of 72) |
Sidechain | 83.9 % (457 of 545) | 79.6 % (262 of 329) | 93.1 % (190 of 204) | 41.7 % (5 of 12) |
Aromatic | 70.7 % (58 of 82) | 70.7 % (29 of 41) | 69.2 % (27 of 39) | 100.0 % (2 of 2) |
Methyl | 100.0 % (90 of 90) | 100.0 % (45 of 45) | 100.0 % (45 of 45) |
1. E3 ubiquitin-protein ligase parkin
MIVFVRFNSS HGFPVEVDSD TSIFQLKEVV AKRQGVPADQ LRVIFAGKEL RNDWTVQNCD LDQQXIVHIV QRPWRKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 300 uM [U-15N] pUBL, 25 mM HEPES, 100 mM sodium chloride, 250 uM TCEP, 200 uM DSS, 300 uM imidazole, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DSS | natural abundance | 200 uM | |
2 | HEPES | natural abundance | 25 mM | |
3 | TCEP | natural abundance | 250 uM | |
4 | imidazole | natural abundance | 300 uM | |
5 | pUBL | [U-15N] | 300 uM | |
6 | sodium chloride | natural abundance | 100 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 300 uM [U-13C] pUBL, 25 mM HEPES, 100 mM sodium chloride, 250 uM TCEP, 200 uM DSS, 300 uM imidazole, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | DSS | natural abundance | 200 uM | |
10 | HEPES | natural abundance | 25 mM | |
11 | TCEP | natural abundance | 250 uM | |
12 | imidazole | natural abundance | 300 uM | |
13 | pUBL | [U-13C] | 300 uM | |
14 | sodium chloride | natural abundance | 100 mM | |
15 | H2O | natural abundance | 90 % | |
16 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 400 uM [U-99% 13C; U-99% 15N] pUBL, 25 mM HEPES, 100 mM sodium chloride, 250 uM TCEP, 200 uM DSS, 300 uM imidazole, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | DSS | natural abundance | 200 uM | |
18 | HEPES | natural abundance | 25 mM | |
19 | TCEP | natural abundance | 250 uM | |
20 | imidazole | natural abundance | 300 uM | |
21 | pUBL | [U-99% 13C; U-99% 15N] | 400 uM | |
22 | sodium chloride | natural abundance | 100 mM | |
23 | H2O | natural abundance | 90 % | |
24 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 400 uM pUBL, 25 mM HEPES, 100 mM sodium chloride, 250 uM TCEP, 200 uM DSS, 300 uM imidazole, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | DSS | natural abundance | 200 uM | |
26 | HEPES | natural abundance | 25 mM | |
27 | TCEP | natural abundance | 250 uM | |
28 | imidazole | natural abundance | 300 uM | |
29 | pUBL | natural abundance | 400 uM | |
30 | sodium chloride | natural abundance | 100 mM | |
31 | D2O | natural abundance | 100 % |