Solution NMR structure of the major species of DANCER-2, a dynamic and natively folded pentamutant of the B1 domain of streptococcal protein G (GB1)
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.1 % (566 of 635) | 85.4 % (275 of 322) | 91.3 % (230 of 252) | 100.0 % (61 of 61) |
Backbone | 99.1 % (333 of 336) | 98.3 % (114 of 116) | 99.4 % (163 of 164) | 100.0 % (56 of 56) |
Sidechain | 81.2 % (285 of 351) | 78.2 % (161 of 206) | 85.0 % (119 of 140) | 100.0 % (5 of 5) |
Aromatic | 47.1 % (32 of 68) | 47.1 % (16 of 34) | 45.5 % (15 of 33) | 100.0 % (1 of 1) |
Methyl | 95.2 % (59 of 62) | 96.8 % (30 of 31) | 93.5 % (29 of 31) |
1. entity 1
MTFKAIINGK TLKGETTTEA VDAATAEKVF KQYFNDNGLD GEWTYDDATK TFTVTESolvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 200 uM [U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | EDTA | natural abundance | 100 uM | |
2 | protein (GB1) | [U-98% 15N] | 200 uM | |
3 | sodium azide | natural abundance | 0.02 % | |
4 | sodium phosphate | natural abundance | 10 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 200 uM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | EDTA | natural abundance | 100 uM | |
6 | protein (GB1) | [U-99% 13C; U-98% 15N] | 200 uM | |
7 | sodium azide | natural abundance | 0.02 % | |
8 | sodium phosphate | natural abundance | 10 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4, Details 200 uM [U-99% 13C; U-98% 15N] protein (GB1), 10 mM sodium phosphate, 100 uM EDTA, 0.02 % sodium azide, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | EDTA | natural abundance | 100 uM | |
10 | protein (GB1) | [U-99% 13C; U-98% 15N] | 200 uM | |
11 | sodium azide | natural abundance | 0.02 % | |
12 | sodium phosphate | natural abundance | 10 mM |