NMR structure of monomeric human IRAK-M Death Domain R56D, Y61E mutant
SEFGSMAGNC GARGALSAHT LLFDLPPALL GELCAVLDSC DGALGWRGLA ERLSSSWLDV DHIEKEVDQG KSGTRELLWS WAQKNKTIGD LLQVLQEMGH RRAIHLITNY GAVLSPSEKS YQEG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.2 % (1249 of 1385) | 87.7 % (626 of 714) | 91.4 % (492 of 538) | 98.5 % (131 of 133) |
Backbone | 97.7 % (721 of 738) | 97.3 % (252 of 259) | 97.8 % (350 of 358) | 98.3 % (119 of 121) |
Sidechain | 84.0 % (636 of 757) | 82.2 % (374 of 455) | 86.2 % (250 of 290) | 100.0 % (12 of 12) |
Aromatic | 42.0 % (42 of 100) | 42.0 % (21 of 50) | 37.0 % (17 of 46) | 100.0 % (4 of 4) |
Methyl | 96.5 % (137 of 142) | 98.6 % (70 of 71) | 94.4 % (67 of 71) |
1. entity 1
SEFGSMAGNC GARGALSAHT LLFDLPPALL GELCAVLDSC DGALGWRGLA ERLSSSWLDV DHIEKEVDQG KSGTRELLWS WAQKNKTIGD LLQVLQEMGH RRAIHLITNY GAVLSPSEKS YQEGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.67, Details 800 uM [U-99% 13C; U-99% 15N] IRAK-M Death Domain_R56D_Y61E, 20 mM sodium chloride, 5 mM TCEP, 10 mM TRIS, 5 mM sodium azide, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | IRAK-M Death Domain_R56D_Y61E | [U-99% 13C; U-99% 15N] | 800 uM | |
2 | TCEP | natural abundance | 5 mM | |
3 | TRIS | natural abundance | 10 mM | |
4 | sodium azide | natural abundance | 5 mM | |
5 | sodium chloride | natural abundance | 20 mM |