Solution structure of VKK38 bound to plasminogen kringle 2
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 68.1 % (327 of 480) | 60.4 % (154 of 255) | 74.7 % (136 of 182) | 86.0 % (37 of 43) |
Backbone | 95.0 % (228 of 240) | 95.1 % (77 of 81) | 95.8 % (114 of 119) | 92.5 % (37 of 40) |
Sidechain | 48.7 % (136 of 279) | 44.3 % (77 of 174) | 57.8 % (59 of 102) | 0.0 % (0 of 3) |
Aromatic | 0.0 % (0 of 24) | 0.0 % (0 of 12) | 0.0 % (0 of 12) | |
Methyl | 63.2 % (24 of 38) | 68.4 % (13 of 19) | 57.9 % (11 of 19) |
1. M protein
GSVKKLNDEV ALERLKNERH VHDEEVELER LKNERHDHDYSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.7, Details 1.0 mM [U-13C; U-15N] VKK38, 2.5 mM Kringle2, 20 mM [U-100% 2H] BisTris-d19, 0.2 mM DSS, 2 mM EDTA, 3 mM sodium azide, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | BisTris-d19 | [U-100% 2H] | 20 mM | |
2 | DSS | natural abundance | 0.2 mM | |
3 | EDTA | natural abundance | 2 mM | |
4 | Kringle2 | natural abundance | 2.5 mM | |
5 | VKK38 | [U-13C; U-15N] | 1.0 mM | |
6 | sodium azide | natural abundance | 3 mM |