BMRB: 30282

Structure and dynamics of RNA repeat expansions that cause Huntington's Disease and myotonic dystrophy type 1

Authors

Chen, J.L., VanEtten, D.M., Fountain, M.A., Yildirim, I., Disney, M.D.

Assembly

RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3')

Entity

1. RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3') (polymer, Thiol state: not present), 15 monomers, 4892.941 × 2 Da Detail

GACCAGCAGC AGGUC

Total weight

9785.882 Da

Max. entity weight

4892.941 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 52.7 %, Completeness (bb): 48.9 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	52.7 % (69 of 131)	52.7 % (69 of 131)
Suger, PO4	48.9 % (44 of 90)	48.9 % (44 of 90)
Nucleobase	61.0 % (25 of 41)	61.0 % (25 of 41)
Aromatic	80.6 % (25 of 31)	80.6 % (25 of 31)

1. entity 1

GACCAGCAGC AGGUC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 100% D2O.

#	Name	Isotope labeling	Type	Concentration
1	EDTA	natural abundance		0.25 mM
2	RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3')	NA Adenosine, Cytidine,Guanosine, Uridine		0.5 mM
3	potassium phosphate	natural abundance		5.0 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Type	Concentration
4	EDTA	natural abundance		0.25 mM
5	RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3')	NA Adenosine, Cytidine,Guanosine, Uridine		0.5 mM
6	potassium phosphate	natural abundance		5.0 mM

Hide sample codition

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 41.2 %, Completeness (bb): 29.4 % Detail

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• CSV
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Polymer type: polyribonucleotide

	Total	1H
All	41.2 % (108 of 262)	41.2 % (108 of 262)
Suger, PO4	29.4 % (53 of 180)	29.4 % (53 of 180)
Nucleobase	67.1 % (55 of 82)	67.1 % (55 of 82)
Aromatic	72.6 % (45 of 62)	72.6 % (45 of 62)

1. entity 1

GACCAGCAGC AGGUC

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 100% D2O.

#	Name	Isotope labeling	Type	Concentration
1	EDTA	natural abundance		0.25 mM
2	RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3')	NA Adenosine, Cytidine,Guanosine, Uridine		0.5 mM
3	potassium phosphate	natural abundance		5.0 mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6, Details 0.5 mM NA Adenosine, Cytidine,Guanosine, Uridine RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3'), 5.0 mM potassium phosphate, 0.25 mM EDTA, 95% H2O/5% D2O.

#	Name	Isotope labeling	Type	Concentration
4	EDTA	natural abundance		0.25 mM
5	RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3')	NA Adenosine, Cytidine,Guanosine, Uridine		0.5 mM
6	potassium phosphate	natural abundance		5.0 mM

Hide sample codition

Release date

2017-04-26

Citation

Structure and dynamics of RNA repeat expansions that cause Huntington's Disease and myotonic dystrophy type 1
Chen, J.L., VanEtten, D.M., Fountain, M.A., Yildirim, I., Disney, M.D.
Biochemistry (2017), 56, 3463-3474, PubMed 28617590 , DOI 10.1021/acs.biochem.7b00252 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. RNA (5'-R(*GP*AP*CP*CP*AP*GP*CP*AP*G)-3'), : 1 : 1 : 4 entities Detail

Experiments performed 2 experiments Detail