BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	98.1 % (869 of 886)	98.7 % (458 of 464)	97.4 % (340 of 349)	97.3 % (71 of 73)
Backbone	97.5 % (431 of 442)	98.7 % (148 of 150)	96.8 % (214 of 221)	97.2 % (69 of 71)
Sidechain	98.8 % (509 of 515)	98.7 % (310 of 314)	99.0 % (197 of 199)	100.0 % (2 of 2)
Aromatic	95.2 % (80 of 84)	95.2 % (40 of 42)	95.2 % (40 of 42)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. entity 1

GEFGSMVKET KFYDILGVPV TATDVEIKKA YRKCALKYHP DKNPSEEAAE KFKEASAAYE ILSDPEKRDI YDQFG

Show sample condition

Sample #1

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

Sample #2

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		4.0 (±0.1) mg/mL
4	NaCl	natural abundance		150 mM

Sample #3

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	Ydj1 protein (1-70 residues)	[U-15N]		5.5 (±0.1) mg/mL
6	NaCl	natural abundance		150 mM

Sample #4

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
7	Ydj1 protein (1-70 residues)	[U-15N]		5.5 (±0.1) mg/mL
8	NaCl	natural abundance		150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.39 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.41 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5VSO, Strand ID: A Detail

Release date

2017-05-16

Citation

Broadening the functionality of a J-protein/Hsp70 molecular chaperone system
Schilke, B.A., Ciesielski, S.J., Ziegelhoffer, T., Kamiya, E., Tonelli, M., Lee, W., Cornilescu, G., Hines, J.K., Markley, J.L., Craig, E.A.
PLoS Genet. (2017), 13, e1007084-e1007084, PubMed 29084221 , DOI 10.1371/journal.pgen.1007084 , Abstract

Related entities 1. Yeast dnaJ protein 1, : 1 : 2 : 286 entities Detail

Interaction partners 1. Yeast dnaJ protein 1, : 879 interactors Detail

More details for 879 interactors identified by 16 citations

Experiments performed 17 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

3 2D 1H-13C HSQC aromatic

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

4 3D HCCH-TOCSY

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

5 3D HBHA(CO)NH

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

6 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		4.0 (±0.1) mg/mL
4	NaCl	natural abundance		150 mM

7 3D 1H-13C NOESY aliphatic

Instrument

Bruker AvanceIII - 900 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

8 3D H(CCO)NH

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

9 3D C(CO)NH

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

10 2D 1H-15N HSQC

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	Ydj1 protein (1-70 residues)	[U-15N]		5.5 (±0.1) mg/mL
6	NaCl	natural abundance		150 mM

11 2D 1H-15N HSQC

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 283 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
5	Ydj1 protein (1-70 residues)	[U-15N]		5.5 (±0.1) mg/mL
6	NaCl	natural abundance		150 mM

12 2D 1H-15N HSQC

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
3	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		4.0 (±0.1) mg/mL
4	NaCl	natural abundance		150 mM

13 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State anisotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 283 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
7	Ydj1 protein (1-70 residues)	[U-15N]		5.5 (±0.1) mg/mL
8	NaCl	natural abundance		150 mM

14 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 283 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
7	Ydj1 protein (1-70 residues)	[U-15N]		5.5 (±0.1) mg/mL
8	NaCl	natural abundance		150 mM

15 3D CBCA(CO)NH

Instrument

Varian VXRS - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

16 3D HNCACB

Instrument

Varian VXRS - 800 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

17 3D HNCO

Instrument

Varian VXRS - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 279 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	Ydj1 protein (1-70 residues)	[U-15N; U-13C]		3.5 (±0.1) mg/mL
2	NaCl	natural abundance		150 mM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_30293_5vso.nef
Input source #2: Coordindates	5vso.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70-----
GEFGSMVKETKFYDILGVPVTATDVEIKKAYRKCALKYHPDKNPSEEAAEKFKEASAAYEILSDPEKRDIYDQFG
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GEFGSMVKETKFYDILGVPVTATDVEIKKAYRKCALKYHPDKNPSEEAAEKFKEASAAYEILSDPEKRDIYDQFG

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	75	0	0	100.0

Content subtype: combined_30293_5vso.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	867		100.0 (chain: A, length: 75)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	OK	1873 (1)	noe	90.7 (chain: A, length: 75)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_3	OK	127 (127)	.	92.0 (chain: A, length: 75)
1	RDC restraints	DYANA/DIANA_dipolar_coupling_5	OK	59 (59)	.	78.7 (chain: A, length: 75)

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 75, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 457
  - ¹³C chemical shifts: 340
  - ¹⁵N chemical shifts: 70
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	464	457	98.5
¹³C chemical shifts	349	340	97.4
¹⁵N chemical shifts	75	70	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	150	147	98.0
¹³C chemical shifts	150	143	95.3
¹⁵N chemical shifts	71	68	95.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	314	310	98.7
¹³C chemical shifts	199	197	99.0
¹⁵N chemical shifts	4	2	50.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	34	34	100.0
¹³C chemical shifts	34	34	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	40	95.2
¹³C chemical shifts	42	40	95.2

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 75, Sequence coverage: 90.7%
- Total number of restraints: 1866
- Weight of restraints: 1.0 (1866)
- Potential type of restraints: upper-bound-parabolic (1866)
- Range of distance target values: 2.2, 8.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 75, Sequence coverage: 92.0%
- Total number of restraints: 127
- Total number of restraints per polymer type: 127
- Weight of restraints: 1.0 (127)
- Potential type of restraints: square-well-parabolic (127)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

RDC restraints

Saveframe: DYANA/DIANA_dipolar_coupling_5

Status: OK
Experiment type: .

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 75, Sequence coverage: 78.7%

--------10--------20--------30--------40--------50--------60--------70-----
GEFGSMVKETKFYDILGVPVTATDVEIKKAYRKCALKYHPDKNPSEEAAEKFKEASAAYEILSDPEKRDIYDQFG
      |||||||||||| ||||||||||||||||||||  |   ||||||||||||||||||| |||||||   
......VKETKFYDILGV.VTATDVEIKKAYRKCALKYH..K...EEAAEKFKEASAAYEILSD.EKRDIYD   
--------10--------20--------30--------40--------50--------60--------70--

Total number of restraints: 59
Potential type of restraints: undefined (59)
Range of observed RDC values: 100.0, -100.0 (Hz)
RDC restraints per residue
- Chain_ID: A
  None

Keywords DnaJ, J-domain, J-protein, chaperone

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Found 1 Document (3.768 seconds)

BMRB: 30293

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Yeast dnaJ protein 1, : 1 : 2 : 286 entities Detail

Interaction partners 1. Yeast dnaJ protein 1, : 879 interactors Detail

Experiments performed 17 experiments Detail

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NMR combined restraints 5 contents Detail