Solution NMR structure of the BRCT domain of S. cerevisiae Rev1
GHMSSKIFKN CVIYINGYTK PGRLQLHEMI VLHGGKFLHY LSSKKTVTHI VASNLPLKKR IEFANYKVVS PDWIVDSVKE ARLLPWQNYS LTS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.5 % (970 of 1148) | 85.6 % (511 of 597) | 80.8 % (366 of 453) | 94.9 % (93 of 98) |
Backbone | 93.6 % (515 of 550) | 92.5 % (173 of 187) | 94.2 % (258 of 274) | 94.4 % (84 of 89) |
Sidechain | 79.0 % (542 of 686) | 82.4 % (338 of 410) | 73.0 % (195 of 267) | 100.0 % (9 of 9) |
Aromatic | 35.1 % (40 of 114) | 63.2 % (36 of 57) | 3.6 % (2 of 55) | 100.0 % (2 of 2) |
Methyl | 93.9 % (107 of 114) | 94.7 % (54 of 57) | 93.0 % (53 of 57) |
1. entity 1
GHMSSKIFKN CVIYINGYTK PGRLQLHEMI VLHGGKFLHY LSSKKTVTHI VASNLPLKKR IEFANYKVVS PDWIVDSVKE ARLLPWQNYS LTSSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 15N] Rev1-BRCT, 50 mM Sodium phosphate buffer, 50 mM NaCl, 90% H2O/10% D2O.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NaCl | natural abundance | 50 mM | |
2 | Rev1-BRCT | [U-100% 15N] | 1 mM | |
3 | Sodium phosphate buffer | natural abundance | 50 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1 mM [U-100% 13C; U-100% 15N] Rev1-BRCT, 50 mM sodium phosphate buffer, 50 mM NaCl, 90% H2O/10% D2O.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | NaCl | natural abundance | 50 mM | |
5 | Rev1-BRCT | [U-100% 13C; U-100% 15N] | 1 mM | |
6 | sodium phosphate buffer | natural abundance | 50 mM |